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- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见包装
- 保质期：
见包装
- 英文名：
X-tremeGENE? 9 DNA Transfection Reagent
- 库存：
999
- 供应商：
博奥派克
- 规格：
1ml
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文献和实验Cationic Liposome-Mediated Transfection with Lipofectin Reagent
(2 ,3 ). Some encouraging DNA-delivery results have been obtained; however, the methodology has had some fundamental diffkulties (4 –8 ). Chief among these is that liposomes do not generally fuse with the target cell surface, but are taken up phagocytically
Procedure for Transfection of Mammalian Cells
in a CO2 incubator until the cells are 70-80% confluent. This will usually take 18-24 h. Prepare the following solutions in 12 x 75 mm sterile tubes: Solution A: For each transfection, dilute 2 μg DNA (plasmid) in 375 μl serum-free IMDM
Transient Transfection and Adrenergic Receptor Promoter Analysis
well for transfection of β 1 -AR gene fragments into adherent cell lines, but is much less effective for transfection of DNA into primary cultures. As noted by other investigators ( 5 ), we have applied FuGENE™ 6 transfection reagent, a nonliposomal lipid compound
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