- ￥5929
- Thermo
- 美国
- 90091
- 2025年07月22日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 保质期：
见产品外包装
- 英文名：
HisPur™ Cobalt Purification Kit, 1mL
- 库存：
需确认
- 供应商：
嵘崴达
- CAS号：
见产品外包装
- 规格：
5 个色谱柱试剂盒
HisPur™ 钴纯化试剂盒，1 mL
货号: 90091
The Thermo Scientific HisPur Cobalt Purification Kit contains 1-ml HisPur spin columns, buffers, and collecting tubes for the purification of polyhistidine-tagged proteins. The HisPur Cobalt Resin in these columns is a tetradentate chelating agarose resin charged with divalent cobalt (Co2+) for obtaining high-purity his-tagged proteins with no metal contamination.
Features of HisPur Cobalt Resin:
• High purity—obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions
• Specificity—cobalt-chelate coordination core binds fewer host protein contaminants, resulting in lower background than nickel resins
• Low metal leaching—no metal contamination in eluted histidine-tagged protein sample
• Versatility—purify proteins under native or denaturing conditions; compatible with Thermo Scientific Pierce Cell Lysis Reagents and a variety of buffer additives
• Cost effective—resin can be reused several times or discarded after single use
• Flexibility—available as bulk resin, kits, predispensed columns, chromatography cartridges and 96-well filter plates
上海嵘崴达实业有限公司，专业经营生命科学领域的科研试剂、仪器和实验室消耗品，
致力于向国内生命科学研究人员提供合格产品和完善服务。
主要经营的试剂品牌：
Roche试剂，部分常用现货，周三定货，2-3周到货。
GE试剂，耗材，部分常用现货，周二，周五定货，3周左右。
SIGMA试剂，周一，五定货，3周左右到货。
THERMO试剂，部分现货，周三定货，2-3周到货。
ABCAM试剂，国内现货三天左右，期货四周左右。
Peprotech试剂，部分常用现货，周三定货，2-3周到货。
罗氏原料，请来电来邮咨询。
OXOID，BD培养基货期八周左右，部分现货。
MCE激动剂，ACROS、ALFA、阿拉丁等生化试剂现货定购。
| 90091 | HisPur™ Cobalt Purification Kit, 1mL | 5 columns |
| 90092 | HisPur Cobalt Purification Kit, 3mL | Kit |
| 90093 | HisPur Cobalt Chromatography Cartridges, 1mL | 5 X 1 mL |
| 90094 | HisPur Cobalt Chromatography Cartridges, 5mL | 2 X 5 mL |
| 90095 | HisPur Cobalt Spin Plates | 2 PLATES |
| 90098 | HisPur Ni-NTA Chromatography Cartridges, 1mL | 5 X 1 mL |
| 90099 | HisPur Ni-NTA Chromatography Cartridges, 5mL | 2 X 5 mL |
| 90100 | iodoTMTzero Label Reagent, 5 x 0.2mg | 1 Kit |
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文献和实验spin, and then added drop wise (randomly around the plate) of the previously plated cell cultures (Figure 1a);2.Forty-eight hours later the cell cultures are washed in cold 1xPBS, then scrapped from the plate and collected in 1ml of cold 1x PBS
for at least 2 hours at RT on a rotating wheel (or overnight at 4°C); Incubate tissues for at least 1 hour at RT in 1ml of PBS-Tr (PBS 1X; 0.3% Triton X-100) (see comment 2); Transfer tissues into a pre-Hybridization Mixture (pHM) by passing
相关专题 【实验原理】 琼脂 糖凝胶电泳与醋酸纤维素薄膜电泳原理基本相同，但兼有分子筛效应，从而使不同分子量大小的DNA片段分离。琼脂糖是从琼脂中提取出来的含有较多的酸根和羟基多糖。回收DNA的方法很多，主要包括DEAE纤维素纸插片电泳法、透析袋电泳洗脱法、槽沟电泳洗脱法、v形槽电泳洗脱法以及低熔点琼脂糖凝胶挖块回收法。本实验采用琼脂糖凝胶DNA片段纯化试剂盒，此试剂盒采用独特的凝胶融解系统，结合DNA制备膜技术，具有高效、快速、方便
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