- ￥6281
- Thermo
- 美国
- 90090
- 2025年07月21日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C
- 保质期：
见产品外包装
- 英文名：
HisPur™ Cobalt Purification Kit, 0.2mL
- 库存：
需确认
- 供应商：
嵘崴达
- CAS号：
见产品外包装
- 规格：
25 个色谱柱试剂盒
HisPur™ 钴纯化试剂盒，0.2 mL
货号: 90090
The Thermo Scientific HisPur Cobalt Purification Kit contains 0.2-ml HisPur spin columns, buffers, and collecting tubes for the purification of polyhistidine-tagged proteins. The HisPur Cobalt Resin in these columns is a tetradentate chelating agarose resin charged with divalent cobalt (Co2+) for obtaining high-purity his-tagged proteins with no metal contamination.
Features of HisPur Cobalt Resin:
• High purity—obtain more than 10 milligrams of pure His-tagged protein per milliliter of resin without optimizing imidazole washing conditions
• Specificity—cobalt-chelate coordination core binds fewer host protein contaminants, resulting in lower background than nickel resins
• Low metal leaching—no metal contamination in eluted histidine-tagged protein sample
• Versatility—purify proteins under native or denaturing conditions; compatible with Thermo Scientific Pierce Cell Lysis Reagents and a variety of buffer additives
• Cost effective—resin can be reused several times or discarded after single use
• Flexibility—available as bulk resin, kits, predispensed columns, chromatography cartridges and 96-well filter plates
Compared to Ni-IDA and tetradentate Ni2+ chelate resins, HisPur Cobalt Resin binds histidine-tagged proteins with higher specificity (less off-target binding) and releases them with gentler conditions (lower concentrations of imidazole). Although Ni(2+) chelate resins achieve high protein yields, they bind somewhat indiscriminantly, resulting in suboptimal purity. Often, additional cleanup steps are required. By contrast, cobalt maximizes protein purity without sacrificing protein yield. HisPur Cobalt Resin binds fewer nonspecific proteins, displays less metal leaching and enables less stringent elution conditions than nickel resins.
上海嵘崴达实业有限公司，专业经营生命科学领域的科研试剂、仪器和实验室消耗品，
致力于向国内生命科学研究人员提供合格产品和完善服务。
主要经营的试剂品牌：
Roche试剂，部分常用现货，周三定货，2-3周到货。
GE试剂，耗材，部分常用现货，周二，周五定货，3周左右。
SIGMA试剂，周一，五定货，3周左右到货。
THERMO试剂，部分现货，周三定货，2-3周到货。
ABCAM试剂，国内现货三天左右，期货四周左右。
Peprotech试剂，部分常用现货，周三定货，2-3周到货。
罗氏原料，请来电来邮咨询。
OXOID，BD培养基货期八周左右，部分现货。
MCE激动剂，ACROS、ALFA、阿拉丁等生化试剂现货定购.
| 90090 | HisPur™ Cobalt Purification Kit, 0.2mL | 25 columns |
| 90091 | HisPur™obalt Purification Kit, 1mL | Kit |
| 90092 | HisPur™ Cobalt Purification Kit, 3mL | Kit |
| 90093 | HisPur™Cobalt Chromatography Cartridges, 1mL | 5 X 1 mL |
| 90094 | HisPur™ Cobalt Chromatography Cartridges, 5mL | 2 X 5 mL |
| 90095 | HisPur™ Cobalt Spin Plates | 2 PLATES |
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文献和实验the collection tubes for next step. 6) Add 700 ul of SPW Wash buffer diluted with ethanol to the HiBind™ MicroElute™ DNA column and spin at 10,000 x g for 1 minute. Discard the flow-through and place the column back into same 2mL collection tube.
from the handbook and annotated based on experience with the kit. Protocol: QIAprep Spin Miniprep Kit Using a Microcentrifuge This protocol is designed for purification of up to 20 μg of high-copy plasmid DNA from 1�5 ml overnight cultures of E. coli in LB
application. 3. RNA Storage Store the purified RNA on ice for immediate use. For long–term storage, keep the purified RNA at –80°C. Perform DNase I treatment after purification (refer to the PureLink™ RNA Mini Kit manual) to assure highly
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