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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
of adherent cells: NP40-1% lysis buffer (50 mM TRIS-HCl pH 8.0, 150 mM NaCl, 1% NP-40) supplemented with a protease inhibitor cocktail tablet (Roche). 8. Identify the protein of interest by immunoprecipitation (IP) with specific antibody. Suggested IP
Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction
?protein interaction is described using an antibody and its antigen as an example. The affinity of the antibody for its antigen is determined by measuring the binding kinetics of the interaction. The protocols are divided into three major steps
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