PSD-95单克隆抗体 WB/IC/IP适用 psd-95

Anti-Post Synaptic Density Protein 95, clone 7E3-1B8

The postsynaptic density (PSD) is an electron dense structure just beneath the postsynaptic membrane. Several functions have been proposed for the PSD including regulating receptor number and clustering, anchoring signal transduction molecules at the synapse and mediating adhesion between the presynaptic and postsynaptic membranes. The most abundant PSD protein is the [alpha] subunit of the type II calcium/calmodulin dependent protein kinase ([alpha]CaMKII). This protein is likely to play a role in the calcium-mediated signal transduction at the synapse that mediates certain forms of synaptic plasticity. Another major PSD protein is PSD-95, a member of the guanylate kinase family (GUK) of proteins.

PSD-95

7E3-1B8

Reacts with recombinant rat PSD-95 and identifies a band at approximately ~100 kDa, corresponding to PSD-95, on western blots of rat brain, mouse brain and bovine brain extracts. Also present in rat brain and mouse brain tissue extracts are bands of approximately 80 kDa and 50 kDa. PSD-95 is believed to initiate NMDA receptor clustering on the post synaptic membrane.

• Immunocytochemistry
• Immunoprecipitation
• Western Blotting

Immunocytochemistry:
A previous lot of this antibody was used in IC.

Immunoprecipitation:
A previous lot of this antibody was used in IP.

Optimal working dilutions must be determined by the end user.

• Human
• Mouse
• Rat

Mouse and rat. Expected to react with human based on sequence homology

IgG2a

Recombinant rat PSD-95

~ 95 kDa

Mouse brain, rat brain tissue

Purified moust monoclonal IgG2a liquid in buffer containing PBS, pH 7.2, with 0.09% sodium azide and 50% glycerol.

Stable for 1 year at -20ºC from date of receipt.

Evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected PSD-95 on 10 µg of Mouse brain lysates.

Neuroscience

Synapse & Synaptic Biology

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Chemicon (Millipore)

Purified

Mouse

1 .0 mg/mL

Monoclonal Antibody