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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
dilution). Multiple ChIP reactions can be performed from one 3C ligation reaction. The same ligation reaction can be split and used for ChIP analyses with different antibodies. 24. Add 4-10 µg of the antibody of choice
from each cell culture condition. • Performing a ChIP using the Normal Rabbit IgG is an effective negative control. • The included anti-RNA polymerase II antibody and GAPDH primers provide a positive control for assay technique and reagent integrity
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