Anti-DNA, single stranded spec

In cultures of breast cancer MB-MDA-468 cells treated with protein kinase inhibitor staurosporine, only cells with chromatin condensation typical of apoptosis were labeled with antibody to ssDNA (Catalog Number MAB3299).

FIGURE 1. Fluorescence microphotographs of MB-MDA-468 cells treated with staurosporine, heated in formamide, stained with MAB3299 (clone F7-26) and counterstained with DNA fluorochrome DAPI. The same field is shown after UV excitation for DAPI (left panel) and visible light excitation for fluorescein-labeled antibody (right panel). Note that only 3 apoptotic cells with chromatin condensed at the nuclear periphery are stained with the Mab. Magnification, x1000

FIGURE. Photomicrographs of small intestine crypts of a mouse treated with hydroxyurea (A) and small intestine crypts of a control animal (B), treated. Sections of formalin-fixed tissues were heated in formamide, stained with MAB3299 (Clone F7-26) and counterstained with hematoxylin. Brown nuclei - antibody stained apoptotic cells with condensed chromatin and fragmented nuclei.

FIGURE. Photomicrographs of thymus of a mouse treated with hydrocortisone (A) and thymus of a control animal (B). Sections of formalin-fixed tissues were heated in formamide, stained with MAB3299 (clone F7-26) and countestained with hematoxylin. Brown nuclei - antibody stained apoptotic cells with condensed chromatin and fragmented nuclei.

Detection of apoptosis in sections of paraffin-embedded tissues. Tissue sections were stained with anti-ssDNA mAb (Catalog Number MAB3299) and hematoxylin. Dark nuclei, antibody-stained apoptotic cells with brown deposits of peroxidase chromagen DAB. Apoptotic cells located in tips of villi in mouse small intestine.

Detection of apoptosis in sections of paraffin-embedded tissues. Tissue sections were stained with anti-ssDNA mAb (Catalog Number MAB3299) and hematoxylin. Dark nuclei, antibody-stained apoptotic cells with brown deposits of peroxidase chromagen DAB. Intense apoptosis is human lung carcinoma.

Detection of apoptosis in sections of paraffin-embedded tissues. Tissue sections were stained with anti-ssDNA mAb (Catalog Number MAB3299) and hematoxylin. Dark nuclei, antibody-stained apoptotic cells with brown deposits of peroxidase chromagen DAB. Microvessel with high rate of apoptosis surrounded with viable tumor cells in human breast carcinoma.

Detection of apoptosis in sections of paraffin-embedded tissues. Tissue sections were stained with anti-ssDNA Mab and hematoxylin. Dark nuclei, antibody-stained apoptotic cells with brown deposits of peroxidase chromagen DAB. (393) Apoptotic cells in human breast carcinoma.

Fluorescence microscopy of apoptotic cells stained with anti-ssDNA MAb. MOLT-4 cultures treated with etoposide were stained with anti-ssDNA Mab F7-26 and DNA fluorochrome DAPI. (394) - UV excitation for DNA fluorochrome. (395) -450-490 nm excitation for fluorescein-labeled antibody. NOTE: only apoptotic cells with condensed and marginated chromatin (394) are stained with the antibody (395).

Fluorescence microscopy of apoptotic cells stained with anti-ssDNA MAb. MOLT-4 cultures treated with etoposide were stained with anti-ssDNA Mab F7-26 and DNA fluorochrome DAPI. (394) - UV excitation for DNA fluorochrome. (395) -450-490 nm excitation for fluorescein-labeled antibody. NOTE: only apoptotic cells with condensed and marginated chromatin (394) are stained with the antibody (395).

Detection of apoptosis in biopsies of human ovarian carcinoma by anti-ssDNA Mab F7-26. (398) Section of tumor fragment incubated in drug-free medium. (399) Section of tumor fragment treated with 6 Ug/mL. cisplatin for 24 hours. NOTE: The number of apoptotic cells with dark nuclei stained by the MAb F7-26 increased after drug treatment.

Detection of apoptosis in biopsies of human ovarian carcinoma by anti-ssDNA Mab F7-26. (398) Section of tumor fragment incubated in drug-free medium. (399) Section of tumor fragment treated with 6 Ug/mL. cisplatin for 24 hours.