- 询价
- Merck millipore
- 中国/美国/德国
- hz-11838-RP02
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温，避光
- 克隆性：
单克隆
- 抗体名：
CD62L / L-Selectin / SELL抗体
免疫原 :
Recombinant Human CD62L / L-Selectin / SELL protein (Catalog#11838-H08H)
Antibody Type : Rabbit Polyclonal Antibody ( Antibody Purification Platform )
抗体宿主 :
Rabbit IgG
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 :
Produced in rabbits immunized with purified, recombinant Human CD62L / L-Selectin / SELL (rh CD62L / L-Selectin / SELL; Catalog#11838-H08H; P14151-1; Met 1-Asn 332). CD62L / L-Selectin / SELL specific IgG was purified by Human CD62L / L-Selectin / SELL affinity chromatography.
CD62L / L-Selectin / SELL抗体Background
L-selectin (SELL), also known asCD62L, is acell adhesion molecule found onleukocytes. It belongs to theselectin family of proteins, which recognise sialylated carbohydrate groups and are involved in the regulation of leukocyte traffic. Selectins are composed of an N-terminal C-type lectin domain, a single EGF domain, a variable number of short consensus repeat (SCR) domains, a transmembrane region and a cytoplasmic tail. L-selectin is the only selectin expressed on leukocytes and mediates a number of leukocyte-endothelial interactions. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules.Ligandspresent onendothelialcells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. L-selectin is commonly found on the cell surfaces of T cells. Naive T-lymphocytes, which have not yet encountered their specific antigen, need to enter secondary lymph nodes to encounter their antigen. Central memory T-lymphocytes, which have encountered antigen, express L-selectin to localize in secondary lymphoid organs.
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文献和实验酸化路易糖X(sLe“)相关的阴离子寡糖序列结合，也可结合硫酸肝素和硫苷脂。CD62L与CD34、G1yCAMl和MAdCAM-1分子上发生岩藻糖化、唾液酸化和硫酸盐化的碳水化合物结合。CD6gL介导白细胞与内皮细胞最初的滞留和滚动，尤其重要的是，CD62L对于未致敏淋巴细胞经HEV归巢到外周淋巴结和派氏集合淋巴结过程中起着重要作用。CD62L还介导白细胞迁移到炎症部位，介导中性粒细胞相互之间的作用。相应配体与CI~2L的结合刺激蛋白酶裂解CD62L，这可能对于维持较高速度的滚动起着重要作用
L-selectin-PNAd Interactions under Flow Conditions.
lower wall or expressed on a cell monolayer. The present protocol will focus on the interactions between L-selectin expressed on neutrophils and PNAd coated onto the plastic surface. For neutrophil purification see Purification of human
人转录因子E2F1 ( E2F1 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 E2F1 单抗包被于酶标板上，标准品和样品中的 E2F1与单抗结合，加入生物素化的抗人 E2F1 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸，在 450nm 处测 OD 值，E2F1 浓度与 OD 值成正比
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