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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温,避光
- 克隆性:
单克隆
- 抗体名:
SELL / CD62L / L-selectin抗体
免疫原 :
Recombinant Mouse SELL / CD62L / L-selectin protein (Catalog#50045-M08H)
Reagents : PE-conjugated rabbit monoclonal antibody
Clone ID :
414
抗体宿主 :
Rabbit IgG
Concentration :
10 μl/Test, 0.1 mg/ml
缓冲液 : Aqueous solution containing 0.5% BSA and 0.09% sodium azide
制备方法 :
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse SELL / CD62L / L-selectin (rM SELL / CD62L / L-selectin; Catalog#50045-M08H; NP_035476.1; Met 1-Asn 332) and conjugated with PE under optimum conditions, the unreacted PE was removed.
SELL / CD62L / L-selectin抗体Background
L-selectin (SELL), also known asCD62L, is acell adhesion molecule found onleukocytes. It belongs to theselectin family of proteins, which recognise sialylated carbohydrate groups and are involved in the regulation of leukocyte traffic. Selectins are composed of an N-terminal C-type lectin domain, a single EGF domain, a variable number of short consensus repeat (SCR) domains, a transmembrane region and a cytoplasmic tail. L-selectin is the only selectin expressed on leukocytes and mediates a number of leukocyte-endothelial interactions. L-selectin acts as a "homing receptor" for leukocytes to enter secondary lymphoid tissues via high endothelial venules.Ligandspresent onendothelialcells will bind to leukocyte expressing L-selectin, slowing leukocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point. L-selectin is commonly found on the cell surfaces of T cells. Naive T-lymphocytes, which have not yet encountered their specific antigen, need to enter secondary lymph nodes to encounter their antigen. Central memory T-lymphocytes, which have encountered antigen, express L-selectin to localize in secondary lymphoid organs.
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文献和实验L-selectin-PNAd Interactions under Flow Conditions.
lower wall or expressed on a cell monolayer. The present protocol will focus on the interactions between L-selectin expressed on neutrophils and PNAd coated onto the plastic surface. For neutrophil purification see Purification of human
CD62E/CD62L/CD62P 分子 CD62E 常用单克隆抗体或代号: 3B7,4D1O;(E- selectin,ELAM- 1) 主要表达细胞: En [AS] 分子质量(kDa)和结构: gp110(C- lectin SF)结合白细胞ELS- 1 功 能: 粘附L- selectin,中性粒细胞通过结合CD15s结合到En CD62L 常用单克隆抗体或代号: Leu8,FMC46;(L-
酸化路易糖X(sLe“)相关的阴离子寡糖序列结合,也可结合硫酸肝素和硫苷脂。CD62L与CD34、G1yCAMl和MAdCAM-1分子上发生岩藻糖化、唾液酸化和硫酸盐化的碳水化合物结合。CD6gL介导白细胞与内皮细胞最初的滞留和滚动,尤其重要的是,CD62L对于未致敏淋巴细胞经HEV归巢到外周淋巴结和派氏集合淋巴结过程中起着重要作用。CD62L还介导白细胞迁移到炎症部位,介导中性粒细胞相互之间的作用。相应配体与CI~2L的结合刺激蛋白酶裂解CD62L,这可能对于维持较高速度的滚动起着重要作用
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