MES PH 6.5; 0.05M-DTT 0.001M S

Tris–盐酸缓冲液（0.05M，25℃）的配制

Tris – 盐酸缓冲液（0.05M ，25℃） 50毫升0.1M三羟甲基氨基甲烷（Tris）溶液与X毫升0.1N盐酸混匀后，加水稀释至100毫升。   pH

常用缓冲溶液常用浓度及pH范围

M r 甘氨酸-盐酸缓冲液（0.05mol/L） 2.2-5.0 甘氨酸M r =75.07

Immobilized pH gradient (IPG) as first dimension

Tris-HCl (50 mM) pH 8.4, urea (6 M), glycerol (30% v/v), SDS (2% w/v) and DTE (2% w/v) for 12 min. -SH groups were subsequently blocked with 100 ml of a solution containing Tris-HCl (50 mM) pH 6.8, urea (6 M), glycerol (30% v/v), SDS (2% w/v