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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商:
上海西宝
- 英文名:
EpiQuik HAT Activity/Inhibition Assay Kit (48 assays)
- 规格:
48次分析
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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
procedure 1. Tissue sections are deparaffinated and bring up to 95% alcohol. 3. Inhibition of endogenous peroxidase 0.3% H2 O2 in methanol for 15 min. 4. Wash in running tap water for 10 min. 5. Wash in PBS (3x5 min). 6. Add
, these assays can readily be performed in a microplate with relatively few cells. Since a cytotoxic factor will reduce the rate of tetrazolium salt cleavage by a population of cells, these metabolic activity assays are frequently used to measure factor-induced
several assays for detecting several kinds of DNA damage (strand breaks, internal crosslinking, DNA/protein crosslinks) and repair activity following exposure to genotoxic agents. The methods include single?cell electrophoresis (comet assay), filter eluting, K
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