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西宝生物
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文献和实验Crystallization of Kinesin Family Motor Proteins
of well solution containing 20-26% PEG2000ME, 0.2 M NaCl, 50 mM HEPES buffer pH 7.0-8.0Yun et al., 2001Ncd 293 - 70018°C17 mg/ml protein in 20 mM HEPES pH 7.4, 200 mM NaCl, 10 mM MgCl2 and 2 mM DTT, pre-incubated with 4 mM AMP PNP or ATP for 2 hCrystals grew in 11
Preparation of Carboxypeptidase Y and Its Properties
Chromatography on DEAE-cellulose: The activated enzyme solution (600ml) is dialyzed against 0.01 M sodium phosphate buffer, pH7.0. Afterwards, add solid NaCl to make the solution 0.1M in NaCl and continue to dialyze until NH 4 + is cleared away
Competent cells, calcium chloride method, E. coli, description
preparation, most of the nucleic acid at this point is RNA). Extract the solution once with 5 ml chloroform:isoamyl solution, centrifuge and save the aqueous layer. Add 0.1 volumes of 3 M Sodium acetate, mix, overlay with 2 volumes of 95% ethanol
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