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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Ethyl Acetate 5,000
- 库存：
大量
- 供应商：
FUJIFILM Wako
- 规格：
1L
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文献和实验PCR clean up using 3M sodium acetate and chilled absolute ethanol
For each 20 ul of PCR product, prepare the following mixture in 1.5 ml Tube. 2 ul of 3M sodium acetate (NaOAc) pH 4.5 40 ul chilled absolute ethanol. Transfer the PCR product (20ul) into the tube containing
Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
and dissolve the precipitate by gentle inversion. 10. Add 4 ml of 4 M Lithium Acetate and incubate on ice for 20 min. 11. Centrifuge at 1,000 X g for 10 min. 12. Transfer the supernatant to a fresh tube, add 16 ml of 100% Ethanol, and incubate
chloroform-isoamyl alcohol (v/v, 24:1). 5. precipitate the DNA with 3-M ammonium acetate and 2.5 volume of 100% ethyl alcohol. 6. wash the DNA pellet with 70% ethanol 7. dry the pellete in speed vacuum centrifuge for 10 minutes.
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