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文献和实验Isolation of a Purified Epithelial Cell Population from Human Colon
. For the human colon, isolation techniques to allow direct study have been described for mononuclear cells in the lamina propria, smooth muscle cells at or below the muscularis mucosae, and cells of the enteric nervous system, located between the subserosa
the opportunity to directly monitor new therapies. In this chapter, we describe a protocol to image, the in vivo distribution of the integrin β7 , expressed by lymphocytes recruited to and retained by the inflamed gut, using a radiolabeled whole antibody
Electron Microscopic Imaging of Integrin
physiologic conformations. To solve this problem, we apply electron tomography to purified integrin, thereby obtaining high-quality three-dimensional (3-D) images that fit well to the atomic structures. We have also taken typical SPA approaches to obtain a 3-D
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