- 询价
- Abcam
- 中国/美国/德国
- xy398304
- 2025年07月06日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
常温，避光
- 克隆性：
单克隆
- 抗体名：
Green Fluorescent Protein (GFP) 抗体
抗原
Green Fluorescent Protein (GFP)
适用
其他选择 Aequorea victoria
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (3H9)
标记
其他选择 非结合性
Green Fluorescent Protein (GFP) 抗体应用范围
其他选择 Western Blotting (WB), 酶联免疫吸附测定（ELISA）
Pubmed 10个引用可参考
规格 100 μL
发货至 中国 (更改)
产品细节
原理 For biochemical analysis of GFP-tagged fusion proteins.
克隆位点 3H9
亚型 IgG2a
特异性 3H9 efficiently recognizes green fluorescent proteins from Aequorea victoria such as eGFP, wtGFP, YFP, or CFP, but no red fluorescent proteins.
产品特性 Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells in particular the prominent green fluorescent protein (GFP) from Aequorea victoria.
Here we offer a rat monoclonal antibody (MAb) against various GFPs, designated as 3H9. The 3H9 MAb is characterized by high affinity and specificity. It is suitable for immunoblotting and ELISA.
With this versatile antibody GFP and fusions thereof become attractive tools for biochemical applications like identification of interacting proteins or Western Blotting.
纯化方法 Hybridoma supernatant. The Hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kDa exclusion membrane to an antibody concentration of 1 mg/ml.
Green Fluorescent Protein (GFP) 抗体目标详细情况
背景 The first green fluorescent protein (GFP) was isolated from Aequorea victoria and has a molecular weight of 27 kDa. Genetic mutations of GFP resulted in fluorescent proteins with different excitation and emission spectra e.g. eGFP, YFP, CFP. Fluorescent proteins became attractive tools for a variety of applications.
研究领域 Tags/Labels
使用细节
应用备注 Western Blot: Used at a dilution of 1/1000. Detects a band of approximately 31 kDa (the predicted molecular weight of GFP).
说明
IF: not working
限制 仅限研究用
贮存及处理
状态 Liquid
浓度 1 mg/mL
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Do not freeze.
储存条件 4 °C
有效期 12 months
Green Fluorescent Protein (GFP) 抗体相关产品如下：xy-2760R Cortactin皮层肌动蛋白抗体
xy-2792R CRHR2促肾上腺皮质释放激素受体2抗体
xy-3241R Phospho-c-Kit(Tyr703)磷酸化原癌基因c-kit抗体
xy-3242R Phospho-c-Kit(Tyr721)磷酸化原癌基因c-kit抗体
xy-2727R CRLF2胸腺基质淋巴细胞生成素受体抗体
xy-4083R phospho-c-Abl (Tyr134)磷酸化非受体酪氨酸激酶c-Abl抗体
xy-4084R phospho-c-Abl (Tyr253)磷酸化非受体酪氨酸激酶c-Abl抗体
xy-5149R CPI17 alpha蛋白磷酸激酶PKC/CPI-17抗体
xy-4085R phospho-c-Abl(Tyr272)磷酸化非受体酪氨酸激酶c-Abl抗体
xy-4086R phospho-c-Abl(Tyr276)磷酸化非受体酪氨酸激酶c-Abl抗体
xy-10088R CLPS胰辅脂酶抗体
xy-9681R C19orf4519号染色体开放阅读框45抗体
xy-9692R C20orf111过氧化氢诱导转录蛋白1抗体
xy-6202R CIKS核因子NFκB激活蛋白1抗体
xy-6987R C12orf23第12号染色体开放阅读框23抗体
xy-6990R γ-Cateninγ-连环素/连接蛋白γ抗体
xy-9767R C10orf410号染色体开放阅读框4抗体
xy-9768R C10orf4710号染色体开放阅读框47抗体
xy-9770R C10orf8110号染色体开放阅读框81抗体
xy-9683R C19orf4719号染色体开放阅读框47抗体
xy-9684R C19orf5419号染色体开放阅读框54抗体
xy-9685R C1GALT1N-乙酰半乳糖胺糖蛋白3、β-半乳糖转移酶1抗体
xy-9677R C19orf1819号染色体开放阅读框18抗体
xy-9678R C19orf2119号染色体开放阅读框21抗体
xy-9679R C19orf2419号染色体开放阅读框24抗体
xy-4851R Collagen alpha-1(II) chain Ⅱ型胶原α1蛋白/软骨钙素抗体
xy-11829R Cortexin 1皮质素1抗体
xy-9666R C8orf708号染色体开放阅读框70抗体
xy-6478R Carboxypeptidase H胰羧肽酶E抗体
xy-5405R phospho-CK18(Ser60)磷酸化细胞角蛋白18抗体
xy-9693R C20orf11220号染色体开放阅读框112抗体
xy-9771R C10orf8810号染色体开放阅读框88抗体
xy-9956R CRCT1富含半胱氨酸C端蛋白1抗体
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体？
检测GFP、EGFP、YFP、EYFP、CFP的GFP抗体GFP是绿色萤光蛋白（Green Fluorescent Protein）的简称，由238个氨基酸残基组成。GFP蛋白质最早是由下村脩等人在1962年在一种学名Aequorea victoria的水母中发现。其基因所产生的蛋白质，在蓝色波长范围的光线激发下，会发出绿色萤光。GFP或其突变体EGFP标签系统等被广泛用于基因表达效率的检测，以及和目的蛋白融合表达用于检测目的蛋白的表达和分布。我们都知道GFP抗体能应用于GFP的表达检测、纯化
Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins
of the jellyfish, Aequoria victoria (1 , 2 ). The original expression plasmid for this jellyfish protein, green fluorescent protein (GFP) yielded a low level of fluorescence. However, through mutagenesis, enhanced GFPs have been produced that are about 35 times
Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos
Green fluorescent protein (GFP) is responsible for the bioluminescence of the Pacific Northwest jellyfish, Aequorea victoria. In A.victoria, the 27-kDa protein absorbs blue light from a photoprotein that is activated by calcium and emits green
技术资料暂无技术资料 索取技术资料
