- 询价
- 钰博生物
- 进口/国产
- yb398304
- 2025年07月16日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 克隆性：
单克隆
- 抗体名：
Green Fluorescent Protein (GFP) 抗体
- 规格：
0.1ml/0.2ml
Green Fluorescent Protein (GFP)
适用
其他选择 Aequorea victoria
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (3H9)
标记
其他选择 非结合性
Green Fluorescent Protein (GFP) 抗体应用范围
其他选择 Western Blotting (WB), 酶联免疫吸附测定（ELISA）
Pubmed 10个引用可参考
规格 100 μL
发货至 中国 (更改)
发货时间 23至28个工作日
产品编号 yb398304
产品细节
原理 For biochemical analysis of GFP-tagged fusion proteins.
克隆位点 3H9
亚型 IgG2a
特异性 3H9 efficiently recognizes green fluorescent proteins from Aequorea victoria such as eGFP, wtGFP, YFP, or CFP, but no red fluorescent proteins.
产品特性 Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells in particular the prominent green fluorescent protein (GFP) from Aequorea victoria.
Here we offer a rat monoclonal antibody (MAb) against various GFPs, designated as 3H9. The 3H9 MAb is characterized by high affinity and specificity. It is suitable for immunoblotting and ELISA.
With this versatile antibody GFP and fusions thereof become attractive tools for biochemical applications like identification of interacting proteins or Western Blotting.
纯化方法 Hybridoma supernatant. The Hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kDa exclusion membrane to an antibody concentration of 1 mg/ml.
目标详细情况
Green Fluorescent Protein (GFP) 抗体背景 The first green fluorescent protein (GFP) was isolated from Aequorea victoria and has a molecular weight of 27 kDa. Genetic mutations of GFP resulted in fluorescent proteins with different excitation and emission spectra e.g. eGFP, YFP, CFP. Fluorescent proteins became attractive tools for a variety of applications.
研究领域 Tags/Labels
使用细节
应用备注 Western Blot: Used at a dilution of 1/1000. Detects a band of approximately 31 kDa (the predicted molecular weight of GFP).
说明
IF: not working
限制 仅限研究用
贮存及处理
状态 Liquid
浓度 1 mg/mL
储存液 Sodium azide
Green Fluorescent Protein (GFP) 抗体注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Do not freeze.
储存条件 4 °C
有效期 12 months
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体？
检测GFP、EGFP、YFP、EYFP、CFP的GFP抗体GFP是绿色萤光蛋白（Green Fluorescent Protein）的简称，由238个氨基酸残基组成。GFP蛋白质最早是由下村脩等人在1962年在一种学名Aequorea victoria的水母中发现。其基因所产生的蛋白质，在蓝色波长范围的光线激发下，会发出绿色萤光。GFP或其突变体EGFP标签系统等被广泛用于基因表达效率的检测，以及和目的蛋白融合表达用于检测目的蛋白的表达和分布。我们都知道GFP抗体能应用于GFP的表达检测、纯化
Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins
of the jellyfish, Aequoria victoria (1 , 2 ). The original expression plasmid for this jellyfish protein, green fluorescent protein (GFP) yielded a low level of fluorescence. However, through mutagenesis, enhanced GFPs have been produced that are about 35 times
Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos
Green fluorescent protein (GFP) is responsible for the bioluminescence of the Pacific Northwest jellyfish, Aequorea victoria. In A.victoria, the 27-kDa protein absorbs blue light from a photoprotein that is activated by calcium and emits green
技术资料暂无技术资料 索取技术资料
