Green Fluorescent Protein (GFP

Green Fluorescent Protein (GFP) 抗体抗原
Green Fluorescent Protein (GFP)
适用
其他选择 Aequorea victoria
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (3H9)
标记
其他选择 非结合性
Green Fluorescent Protein (GFP) 抗体应用范围
其他选择 Western Blotting (WB), 酶联免疫吸附测定（ELISA）
Pubmed 10个引用可参考
规格 100 μL
发货至 中国 (更改)
发货时间 23至28个工作日
产品编号 yb398304
产品细节
原理 For biochemical analysis of GFP-tagged fusion proteins.
克隆位点 3H9
亚型 IgG2a
特异性 3H9 efficiently recognizes green fluorescent proteins from Aequorea victoria such as eGFP, wtGFP, YFP, or CFP, but no red fluorescent proteins.
产品特性 Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells in particular the prominent green fluorescent protein (GFP) from Aequorea victoria.
Here we offer a rat monoclonal antibody (MAb) against various GFPs, designated as 3H9. The 3H9 MAb is characterized by high affinity and specificity. It is suitable for immunoblotting and ELISA.
With this versatile antibody GFP and fusions thereof become attractive tools for biochemical applications like identification of interacting proteins or Western Blotting.
纯化方法 Hybridoma supernatant. The Hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kDa exclusion membrane to an antibody concentration of 1 mg/ml.
目标详细情况
Green Fluorescent Protein (GFP) 抗体背景 The first green fluorescent protein (GFP) was isolated from Aequorea victoria and has a molecular weight of 27 kDa. Genetic mutations of GFP resulted in fluorescent proteins with different excitation and emission spectra e.g. eGFP, YFP, CFP. Fluorescent proteins became attractive tools for a variety of applications.
研究领域 Tags/Labels
使用细节
应用备注 Western Blot: Used at a dilution of 1/1000. Detects a band of approximately 31 kDa (the predicted molecular weight of GFP).
说明
IF: not working
限制 仅限研究用
贮存及处理
状态 Liquid
浓度 1 mg/mL
储存液 Sodium azide
Green Fluorescent Protein (GFP) 抗体注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Do not freeze.
储存条件 4 °C
有效期 12 months