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- Abcam
- 中国/美国/德国
- xy10395-RP03
- 2025年07月10日
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- 文献和实验
- 技术资料
- 保存条件：
常温，避光
- 克隆性：
单克隆
- 抗体名：
IL6 / IFNB2抗体
Ig Type : Rabbit IgG
缓冲液 : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
制备方法 : Produced in rabbits immunized with purified, E.coli-derived, recombinant human IL6 ( rhIL6; Catalog#10395-HNAE; Val 30 - Met 212; NP_000591.1 ). IL6 specific IgG was purified by human IL6 affinity chromatography.
IL6 / IFNB2抗体 Background
Interleukin 6 (IL-6) is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. IL-6 is a cytokine with a wide variety of biological functions and belongs to the IL-6 superfamily. IL-6 is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. The role of IL-6 as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1Ra and IL-10. IL-6 is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Inhibitors of IL-6 are used to treat postmenopausal osteoporosis. Il-6 is thought to be a reason why obese individuals have higher endogeneous levels of CRP. Intranasally administered IL-6 was shown to improve sleep-associated consolidation of emotional memories.
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xy-0283xy OVA (ovalbumin)鸡卵白蛋白
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xy-0891xy Pokemon扑克蒙蛋白
xy-4867xy p70 S6 kinase beta核糖体蛋白S6激酶1
xy-0362xy Protein A蛋白A
xy-0563xy P-GP(P-glycoprotein)P-糖蛋白抗原
xy-3361xy Phospho-RIP2 (Ser176)磷酸化受体结合丝氨酸苏氨酸激酶2
xy-4545xyB Ractopamine/BSA莱克多巴胺与牛血清白蛋白偶联物
xy-4545xyK Ractopamine/KLH莱克多巴胺与血蓝蛋白偶联物
xy-2372xy rHBsAg 重组乙肝病毒表面抗原
xy-0295xy-AF647 Rabbit IgG/Alexa Fluor 647Alexa Fluor 647标记兔IgG(细胞流式同型对照)
xy-2037xyK streptomycin/KLH链霉素偶联血蓝蛋白
xy-5029xy SAHHS腺苷L-同型半胱氨酸水解酶
xy-3857xy SSDD/Steroid sulfatase类固醇硫酸酯酶
xy-2037xyB streptomycin/BSA链霉素偶联牛血清白蛋白
xy-0065xy SP (Substance P)P物质（抗原）
xy-3970xy SDHA琥珀酸脱氢酶复合体亚基A
xy-1175xy SHBG性激素结合球蛋白多肽
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xy-0437xy Streptavidin, SA链霉亲和素
xy-2410xy SHBG性激素结合球蛋白多肽
xy-0726xy Survivin细胞凋亡抑制因子4（抗原）
xy-2037xyA streptomycin/OVA链霉素偶联鸡卵白蛋白
xy-0291xy THY (thyroglobulin,TG)甲状腺球蛋白
xy-0973xyB Tetracycline/BSA四环素偶联牛血清白蛋白
xy-6190xy Thyroxine Binding Globulin甲状腺素结合球蛋白抗原
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xy-0357xy TRH (Thyrotropin releasing hormone )促甲状腺素释放激素
xy-0392xy Thymopentin胸腺五肽
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xy-0387xy rh-TNF-Alpha(rh-Tumor Necrosis Factor alpha)重组人肿瘤坏死因子
xy-1411xy TERT端粒酶逆转录酶
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文献和实验牛白介素 6（IL6）酶联免疫吸附检测试剂盒预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定牛血清、血浆、组织匀浆、细胞裂解液、细胞培养上清液和其他生物液体中 IL6 含量。试剂盒内容试剂名称数量试剂名称数量96孔板(预包被)196孔板覆膜2标准品2标准品稀释液1×20mL检测溶液A1×120μL检测稀释液A1×12mL检测溶液B1×120μL检测稀释液B1×12mLTMB底物1×9mL终止液1×6mL浓洗涤液(30×)1×20mL使用说明书1需自备的设备及试剂1.450±10nm 滤光
1, CALM2, CALM3, CAMK2B, CAMK4, CNR1, ITK, MAP2K7, NCK2, PAK1, PPIA, PPP3CB, PPP3CC, PPP3R1, TRPV6, VAV1, VAV2, VAV3. Ligands: CCL2, CSF2, CSF3, IFNA1, IFNB1, IFNG, IL1A, IL1B, IL2, IL3, IL4, IL6, IL8, IL10, IL12A, IL12B, IL13, IRF1, LTA, TGFB1, TNF
数为 4 X 106，一般会选择 10 cm 直径的培养皿进行细胞培养（80～90% 汇合率）。为了帮助细胞计数，可以再多培养一皿细胞，进行每一皿细胞数目的估测。甲醛是常用的细胞固定剂，可造成蛋白质与 DNA 之间的共价交联，并且这种交联是可逆的。务必使用在有效期内、正确避光保存的分析级或分子生物学级别的甲醛（Formaldehyde，甲醛饱和水溶液，浓度 37%），用培养基稀释到 1%（即稀释 37 倍）。固定条件通常为室温固定 10～15 分钟，时间不宜过长，避免固定过度。甲醛的交联反应通过加入甘氨
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