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- 英文名:
1.2ml 12-Strip tubes,Non-sterile,960 tubes
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- 供应商:
上海古朵生物有限公司
品名: 1.2ml 12-Strip tubes,Non-sterile,960 tubes(耗材与配件、溶液) 1.2ml带盖可拆圆底收集板 货号:SSI-1770-00 品牌:Omega
购买:1.2ml 12-Strip tubes,Non-sterile,960 tubes(耗材与配件、溶液) 我们免费赠送说明书,使用前请,请仔细细阅读,也可来电询问!
本产品仅用于科研实验,不用于临床诊断!
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文献和实验Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
).remove and save polylysine.wash plates 2x with 10ml sterile water.Seed cells: 1/2 X split from confluent plate (approximately 2-5 x 106 cells per 100mm plate) in PC12 culture media.Incubate overnight in 10% CO2 incubator.Cells should be 50-70% confluent
1. Nerve growth factor (2.5S NGF, Bioproducts for Science). Prepare 1 mg/ml stock in 50 mM NaAc, pH 5.0. Sterile filter and store as 5 ul aliquots in 15 ml conical tubes at -80o C. 2. NGF medium: Add 1 ml of F12K medium to each tube of NGF aliquot
Broth culture of E. coli Tubes of nutrient broth (9.9 ml each) Nutrient agar plates Sterile transfer pipettes (1.0 ml) Quebec colony counter (Optional) Procedure 1. Obtain a broth culture of E. coli and carefully mix
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