【产品形式】:艾美捷为您提供的泛素蛋白 propargylamide形式为Lyophilised. Dissolve initially in minimal volume of DMSO then dilute in required aqueous buffer.
【背景资料】:Ubiquitin propargylamide (Ub-PA) is a newly developed potent and specific deubiquitinating enzyme (DUB) inhibitor. Acts as a DUB active site specific probe facilitating detection of DUBs in biological samples. Utility in the stabilisation of ubiquitin conjugates in cell lysates and tissue extracts and as a control inhibitor for the testing and characterisation of potential DUB activity modulators. Ub-PA possess two unique capabilities: 1) Forms a covalent linkage with (the active site Cys residue of) a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses2) Targets all three major DUB families: UCH, USP and OTU