Cal-520 钙检测试剂盒 *去除培养基 尤其适合于难检测

【产品形式】：艾美捷为您提供的Cal-520 钙检测试剂盒 *去除培养基 尤其适合于难检测细胞系*Cal-520 No Wash Calcium Assay Kits provide homogeneous fluorescence-based assays for detecting intracellular calcium mobilization. Cal-520 AM is a new colorless fluorescent calcium-sensitive dye with a significantly improved signal to noise ratio and intracellular retention. Cells expressing a GPCR or calcium channel of interest that signals through calcium are pre-loaded with Cal-520 AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Cal 520 AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells. Its fluorescence is greatly enhanced upon binding to calcium. When cells stimulated with agonists, the receptor signals the release of intracellular calcium, which significantly increase the fluorescence of Cal-520. The characteristics of its long wavelength, high sensitivity, and >100 times fluorescence enhancement, make Cal-520 AM an ideal indicator for the measurement of cellular calcium. The high S/N ratio and better intracellular retention make the Cal-520 calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists. Cal-520 No Wash Calcium Assay Kits provide an optimized assay method for monitoring the GPCRs and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation.

【背景资料】：Cal-520荧光染料为高通量检测细胞内钙离子流动提供了荧光分析方案。作为一款新的钙敏感的荧光染料，Cal-520 AM与目前的绿色钙离子指示剂，如Fluo-3 AM and Fluo-4 AM相比，具有更高的信噪比和细胞内留存。进入表达GPCR或者钙离子通道蛋白的细胞在加入Cal-520 AM后，后者由于具有疏水性的AM基团可以轻易穿透活细胞膜。当其进入到细胞质后，酯酶会将其水解为Cal-520留在细胞内，与细胞内钙离子结合后的Cal-520发出强绿色荧光（荧光强度增强100倍）。由于Cal-520的长波段覆盖、高灵敏度和超强激发，再加上很高的信噪比与细胞内留存，使其成为评估GPCR和钙离子通道研究的卓越工具，也可以用于其拮抗剂和抑制剂的筛选。

美国AAT Bioquest Inc.(前身是ABD Bioquest，Inc.)是一家为从事生命科学研究、诊断研发及药物开发的科学家研发、生产和销售生物分析研究试剂和试剂盒的公司。公司致力于光谱学检测领域，包括显色、荧光和生物发光技术。AAT Bioquest的产品帮助全世界的科学家和生物医药研究者更好的了解生物化学，免疫学，细胞生物学和分子生物学等领域。作为AAT Bioquest Inc.的中国区域代理，艾美捷科技为中国客户提供光谱学检测技术并应用于生化、生理代谢和细胞分析领域的产品，包括显色，荧光和发光技术等全系列解决方案。

点击Cal-520 钙检测试剂盒 *去除培养基 尤其适合于难检测细胞系*查看更详细产品说明，更多应用、储存、价格、货期等信息请致电垂询AAT Bioquest中国区域授权总代理艾美捷科技有限公司。