CA+试剂盒，用于样本中钙含量检测，Calcium Cont

CheKine™ 微量钙含量检测试剂盒（CA⁺试剂盒）

货号：KTB1117-E
产品类型：细胞代谢检测试剂盒

产品概述

CheKine™ 微量钙含量检测试剂盒（Micro Calcium Content Assay Kit）是一款基于比色法原理开发的、用于精准定量检测多种样本中钙离子含量的核心工具。该试剂盒适用于动植物组织、细胞裂解液、血清（浆）及其他液体样本中的钙含量分析，可满足细胞代谢研究及相关生命科学领域对钙信号调控机制深入探索的需求。

检测原理

钙离子在碱性条件下与邻`甲`酚酞络合酮发生特异性反应，生成稳定的紫色复合物。该复合物在575 nm波长处具有特征吸收峰，通过测定其吸光度值即可实现对钙离子含量的准确量化。

组分清单

• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard（钙标准品）

特点与优势

• 广泛的样本兼容性：经验证，适用于细胞、组织、血清等多种样本类型；
• 操作流程简便：优化的反应体系，步骤清晰，易于上手；
• 高灵敏度与稳定性：显色稳定，重复性好，结果可靠。

应用方向

• 细胞代谢研究中钙离子动态监测；
• 骨骼疾病、内分泌失调等相关机制的分子研究；
• 药物筛选及毒性评价中的钙信号通路分析。

保存与运输条件

保存条件：4℃避光保存，有效期6个月。
运输方式：蓝冰冰袋运输，确保试剂稳定性。

注意事项

• 推荐使用新鲜样本，若需保存，请于-80℃冻存（可放置数周）。解冻过程需严格控制温度与时间，室温解冻应在4小时内完成；
• 实验前请确保所有试剂及设备处于适宜温度，以保证检测准确性。

规格与定价

规格	价格（元）	货期
48 T/48 S	99	现货（次日发货）
96 T/96 S	129	现货（次日发货）

警告：本产品仅供科研使用，不适用于人类或临床诊断。用户应确保应用行为不违反相关专利或许可协议，厂家不对因使用本产品导致的专利侵权或其他违规行为承担责任。

背景信息

钙离子（Ca²⁺）作为哺乳动物体内含量最丰富的矿物质元素，是细胞内至关重要的第二信使，参与调控肌肉收缩、细胞增殖、信号转导、神经传递及骨骼维持等多种生理过程。血清钙可分为游离钙、蛋白结合钙和复合钙三种形态，其稳态失衡与多种疾病密切相关。本试剂盒为科研人员提供了一种高效、准确的钙含量检测工具，助力于更深层次的机制研究。

别名：钙离子检测试剂盒、可溶性钙含量测定试剂盒
搜索关键词：钙、钙含量、钙离子、细胞代谢、CheKine

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Calcium Content Assay Kit (Ca²⁺ Kit)

Catalog Number: KTB1117-E
Product Type: Cell Metabolism Assay Kit

Product Overview

The CheKine™ Micro Calcium Content Assay Kit is a core tool developed based on colorimetric principles for accurately quantifying calcium ion content in various samples. This kit is suitable for the analysis of calcium levels in animal and plant tissues, cell lysates, serum (plasma), and other liquid samples, meeting the needs of cell metabolism research and related life sciences fields for in-depth exploration of calcium signaling regulatory mechanisms.

Assay Principle

Under alkaline conditions, calcium ions specifically react with o-cresolphthalein complexone to form a stable purple complex. This complex has a characteristic absorption peak at 575 nm wavelength, and the calcium ion content can be accurately quantified by measuring its absorbance.

Component List

• Reagent I
• Reagent Ⅱ
• Reagent Ⅲ
• Standard (Calcium Standard)

Features and Advantages

• Broad Sample Compatibility: Validated for use with various sample types including cells, tissues, and serum;
• Simple Operation Process: Optimized reaction system with clear steps and user-friendly protocol;
• High Sensitivity and Stability: Stable color development, good reproducibility, and reliable results.

Application Directions

• Dynamic monitoring of calcium ions in cell metabolism studies;
• Molecular research on mechanisms related to bone diseases, endocrine disorders, etc.;
• Analysis of calcium signaling pathways in drug screening and toxicity evaluation.

Storage and Shipping Conditions

Storage Conditions: Store at 4°C protected from light; valid for 6 months.
Shipping Method: Shipped with blue ice packs to ensure reagent stability.

Precautions

• Fresh samples are recommended. If storage is necessary, freeze at -80°C (can be stored for several weeks). The thawing process must strictly control temperature and time; thawing at room temperature should be completed within 4 hours;
• Before the experiment, ensure all reagents and equipment are at appropriate temperatures to guarantee assay accuracy.

Specifications and Pricing

Specification	Price (¥)	Availability
48 T/48 S	99	In stock (ships next day)
96 T/96 S	129	In stock (ships next day)

Warning: This product is for research use only and is not intended for human or clinical diagnostic use. Users should ensure that their application does not violate relevant patents or licensing agreements. The manufacturer is not liable for patent infringement or other violations resulting from the use of this product.

Background Information

Calcium ions (Ca²⁺), as the most abundant mineral element in mammals, are crucial intracellular second messengers involved in regulating various physiological processes such as muscle contraction, cell proliferation, signal transduction, neurotransmission, and bone maintenance. Serum calcium can be divided into three forms: free calcium, protein-bound calcium, and complexed calcium. The imbalance of its homeostasis is closely related to various diseases. This kit provides researchers with an efficient and accurate tool for calcium content detection, aiding in deeper mechanistic studies.

Alias: Calcium Ion Assay Kit, Soluble Calcium Content Determination Kit
Search Keywords: Calcium, Calcium Content, Calcium Ions, Cell Metabolism, CheKine

Note: The above information may change due to product optimization and upgrades. For specific details, please consult Applygen Technologies Inc.