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- Montana Molecular
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- MTA-U0600G-010
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4°C.
- 保质期：
1年
- 英文名：
G-GECO Calcium Assay
- 库存：
100
- 供应商：
艾美捷科技
G-GECO钙含量测定，G-GECO Calcium Assay，G-GECO钙含量测定,G-GECO Calcium Assay
产品名称：绿色PIP2测定-G-GECO Calcium Assay
产品货号：MTA-U0600G-010
产品规格：10mL
测量钙信号动力学，筛选GPCR的Gq活性，以及更多的活细胞荧光检测。将绿色荧光G-GECO钙传感器添加到细胞中，第二天在自动酶标仪或荧光成像系统上测量稳健的信号。该检测试剂在BacMam中进行遗传编码和包装，可在大多数细胞类型中进行有效转导-包括在HEK 293细胞中的均匀和接近100%的效率以及在许多原代或iPSC衍生细胞中的表达。该试剂盒包括G-GECO传感器BacMam、500 mM丁酸钠水溶液以及阳性对照受体和激动剂（hM 1 R BacMam和25 mM卡巴胆碱水溶液）。30 mL和60 mL的尺寸也可用。其他Montana分子工具，包括BacMam包装的GPCR或离子通道，可以很容易地与该检测试剂盒共表达。
Measure Calcium signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the green fluorescent G-GECO Calcium sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the G-GECO sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 30mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs or ion channels can easily be co-expressed with this assay.
4°C.
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文献和实验Deorphanizing G Protein-Coupled Receptors by a Calcium Mobilization Assay
to their activating ligand(s) in high-throughput format (reverse pharmacology). Many of these technologies monitor important steps in the GPCR activation cycle such as the accumulation of secondary messenger molecules (e.g., cAMP, calcium
Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G-Proteins in Arabidopsis
via changes in the level of cytosolic free calcium concentration ([Ca2+ ]cyt ). Various techniques have been developed to measure the change of [Ca2+ ]cyt , e.g., calcium-sensitive microelectrodes, chemical fluorescent dyes, and biosensors based on luminescent
Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay
Many cell surface receptors activate phosphoinositidase(s) C, via G proteins that catalyze the hydrolysis of phosphatidylinositol 4,5-biphosphate to produce the second messengers, inositol(1,4,5)trisphosphate [Ins(1,4,5)P3
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