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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
西宝生物
- 保存条件:
ROOM TEMPERATURE
- 英文名:
ECOLUME™LIQUID SCINTILLATION COCKTAIL
- 库存:
4x1 gal
- 规格:
RADIOCHEMICALS
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文献和实验The ribonuclease protection assay (RPA)
in the scintillation counter. Expect a maximum yield of 1-3 x 106 Cherenkov counts / µl (measurement of cpm / µl without the presence of scintillation fluid) with an acceptable lower limit of 3 x 105 Cherenkov counts / µl. Store the probe at -20°C until needed
实验试剂 1. Aluminum Foil 2. Liquid Nitrogen 3. Dry Ice 4. Phosphate Buffered Saline 5. Protease Inhibitor Cocktail (Sigma Cat.no. P-8340)
is separated from 14C Cm by phase extraction. ASSAY Make up cocktail, keep on ice; does 10 assays 0.25 M Tris-Cl pH 8.0 0.7 ml (0.07/assay) 14C Cm (57µCi/µmol; 25µCi/ml) 0.1 ml (10µl/assay) Bu CoA (5mg/ml) 0.2 ml (20µM/assay) Total 1.0 ml (100µl/assay
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