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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
ROOM TEMPERATURE
- 供应商：
西宝生物
- 库存：
1 gal
- 英文名：
CYTOSCINT™-ESLIQUID SCINTILLATION COCKTAIL
- 规格：
RADIOCHEMICALS
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文献和实验The ribonuclease protection assay (RPA)
in the scintillation counter. Expect a maximum yield of 1-3 x 106 Cherenkov counts / µl (measurement of cpm / µl without the presence of scintillation fluid) with an acceptable lower limit of 3 x 105 Cherenkov counts / µl. Store the probe at -20°C until needed
is separated from 14C Cm by phase extraction. ASSAY Make up cocktail, keep on ice; does 10 assays 0.25 M Tris-Cl pH 8.0 0.7 ml (0.07/assay) 14C Cm (57µCi/µmol; 25µCi/ml) 0.1 ml (10µl/assay) Bu CoA (5mg/ml) 0.2 ml (20µM/assay) Total 1.0 ml (100µl/assay
Measurement of Carbon Fixation Rates in Leaf Samples
a fume hood when working with scintillation fluid or any organic solvent, and work behind the glass shield. The scintillation "cocktail", as it is called, is usually delivered by means of a repipet. The repipet should be calibrated to deliver 5-6 ml
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