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- 英文名：
Hygromycin B
- CAS号：
31282-04-9
Antibiotic
Hygromycin B is an aminocyclitol antibiotic which specifically inhibits protein synthesis in both prokaryotes, eukaryotes and animal cells. Hygromycin B is used as a cell screening antibiotic of eukaryotic cells as well as prokaryotic cells. This selection market is used especially for transformation of plant cells.
[Specification of Hygromycin B]Solubility: Soluble in H2O and methanol (10g/L).pH at 25C (25 g/L): 9.5~12.5Potency: min. 1,000 units/mg
[References]
- Gritz, L. and Davies, J.: Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae, Gene, 25, 179-188 (1983).
- Gonzales, R., et al.: Transformation of the Dermatophyte Trichophyton mentagrophytes to Hygromycin B Resistance, Infect. Immun., 57, 2923-2925 (1989).
- Mohr, G.: Rapid detection of bacterial hygromycin B phosphotransferase in Aspergillus niger transformants, Appl. Microbiol. Biotechnol., 30, 371-374 (1989)
| 产品编号 | 产品名称 | 应用 | 规格 |
| 084-07681 | 50mg/ml Hygromycin B Solution | 生化学 | 20ml |
| 080-07683 | 50mg/ml Hygromycin B Solution | 生化学 | 100ml |
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文献和实验，不溶于非极性溶剂。对金葡菌209P、肺炎杆菌与人型结核杆菌的最小抑菌浓度MIC为25、12.5与1.56μg/ml，对沟端螺旋体、支原体亦有作用。实验室 用的潮霉素一般为潮霉素B(Hygromycin B)。 潮霉素B在20世纪50年代被发现，当时用作动物用药。而现在它仍然作为驱虫抗虫药被加入鸡和猪的饲料中。用于产生潮霉素的吸水链霉菌在1953年被首次从土壤中分离并获得纯培养 。潮霉素B相关的抗性基因发现于20世纪80年代早期。 潮霉素B与大多数氨
of a gene from a diploid organism, a wild-type strain of the Xanthophyllomyces dendrorhous yeast, using hygromycin B resistance as the only selection marker. To accomplish this, in a first step, a heterozygous hygromycin B-resistant strain is obtained
-411). Among these three selection systems, only hygromycin B resistance provides a dominant selection that can be used for any wild type strain. A second dominant selection marker is needed to transform strains that are already hygromycin B resistant
