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BAMBANKER®
无血清细胞冻存液
| BAMBANKER® 是一种无血清细胞冻存液。 |
 |
◆产品特性
● 即用型细胞冻存液
● 无需分步降温，直接使用
● 无需稀释
● 无需程序降温盒
● -80℃ 长期保存
● 无血清
◆无血清冻存液的优点
● 与含血清类型相比，批次间的成分组成差异小，可保持稳定的品质。
● 不含血清，因此没有因动物源的未知成分和感染物质所产生的影响与风险。
● 可对无血清驯化细胞进行冷冻，节省再驯化步骤。
◆操作流程
1）收集生长对数期*的细胞（5×105-1×107个细胞）
2）用１mL 该细胞冻存液悬浮细胞，置于冻存管中，不需预冷，直接 -80℃ 冷冻保存，
也可在－80℃冻存 12 小时后转移至液氮中保存。
3）用恒温箱或者水浴锅快速复苏细胞
*冷冻细胞必须处于生长对数期
◆无菌检测**
内毒素：生色底物法
支原体：荧光抗体法
真菌和细菌：依据日本药典
（**：可索取检验证书）
BAMBANKER® Direct
BAMBANKER Direct 是“无血清型”细胞冻存液。
◆BAMBANKER® Direct无需离心收集细胞
① 不需冻存前预处理，操作简便
② 不需稀释，直接使用
③ 无需分步降温，直接使用
④ 可快速、长期冻存细胞（-80℃或液氮）
⑤ 不含血清
◆BAMBANKER® Direct冻存步骤VS常规冻存步骤
使用本产品无需经过离心等复杂步骤，只需往培养基内添加与培养液等量的 BAMBANKER Direct，再分装到冻存管，置于-80℃ 便可冻存细胞。
◆应用
【BAMBANKER使用例】
| 细胞名称 | 保存时间 | 生存率 | ||
| BAMBANKER | 公司A（含血清) | 公司A（不含血清） | ||
| P3U1 （小鼠骨髓瘤细胞系） |
12个月 | 95% | 95% | 70% |
| K562 （人白血病细胞系） |
12个月 | 73% | 70% | 60% |
| 人体胃黏膜上皮细胞 | 10个月 | 100% | 62% | 56% |
| human γδT cells （人γδT细胞） |
10个月 | 65% | 37% | 35% |
| Daudi （人 B细胞系） |
12个月 | 100% | 100% | 92% |
| PC12 （大鼠源肾上腺嗜铬细胞瘤） |
11个月 | 95% | 59% | 20% |
| human B cell line （人B细胞系） |
9个月 | 74% | 54% | 35% |
| OKT4 （小鼠杂交瘤细胞） |
12个月 | 100% | 100% | 92% |
| B细胞系 (猴） |
10个月 | 56% | 40% | 18% |
【低温冻存实验证明以下细胞保存完好】
| 3T3‐L1（小鼠前脂肪细胞系） |
A431（人扁平上皮癌细胞系） |
BAEC（牛主动脉血管内皮细胞系） |
| Balb/3T3（小鼠成纤维细胞系） |
C2C12（小鼠骨骼肌细胞系） |
Daudi（人B细胞系） |
| ECV304（人脐静脉内皮细胞系） |
H295R（肾上腺皮质细胞） |
HEK293（人胚胎肾细胞系） |
| HEK293T（人胚胎肾细胞系） |
HeLa（人子宫颈癌细胞系） |
HeLa S3（人子宫颈癌细胞系） |
| HepG2（人肝癌细胞系） |
HFF（人正常成纤维细胞系） |
Huh7（人肝癌细胞系） |
| Jurkat（人白血病T细胞系） |
K562（人慢性骨髓性白血病细胞系） |
KATOIII （人胃癌上皮细胞系） |
| KLM‐1（人胰腺癌细胞系） |
MDCK（犬肾小管上皮细胞系） |
MEF（小鼠胚胎成纤维细胞） |
| NIH3T3（小鼠胚胎皮肤细胞） |
OKT4（小鼠杂交瘤细胞） |
OP9（小鼠骨髓基质细胞） |
| P3U1（小鼠骨髓瘤细胞系） |
PANC‐1（人胰腺癌细胞系） |
PC12（大鼠源肾上腺嗜铬细胞瘤） |
| RPE（人视网膜上皮细胞系） |
SNL（小鼠胚胎成纤维细胞） |
TSU‐Pr1（人前列腺癌细胞系） |
| Vero（非洲绿猴肾细胞系） |
human γδT cells （人γδT细胞） |
human B cell line （人B细胞系） |
| HDF（人皮肤成纤维细胞） | HCC20（人乳腺原发性导管癌细胞） | BMMCs（人骨髓单核细胞系） |
| BMMCs（猪骨髓单核细胞系） | BMSCs（马骨髓间充质干细胞系） | C1（人成纤维细胞系） |
| CEF（牛胚胎成纤维细胞） | CHO-K1（中国仓鼠卵巢细胞系） | DPCs（大鼠牙髓细胞） |
| DPCs（人牙髓细胞） | ESCs（人胚胎干细胞） | EVT（人绒毛外滋养层细胞） |
| GH3（大鼠垂体瘤细胞） | Gli36（胶质瘤细胞系） | h1（人类胚胎干细胞） |
| h9（人类胚胎干细胞） | HN4（人口腔上皮细胞系） | HS-RMS-2 （多形性横纹肌肉瘤细胞系） |
| IPS（人诱导性多能干细胞） | LNCaP clone FGC （人前列腺癌细胞） |
MCF 10A（人正常乳腺细胞） |
| MEF-BL/6-1 （小鼠胚胎成纤维细胞） |
MNCs（人单核细胞） | MSCs（大鼠间充质干细胞系） |
| PBMCs（人外周血单个核细胞） | PDL（人牙周膜细胞） | pES（大鼠孤雌胚胎干细胞系） |
| Sf9（草地贪夜蛾细胞系） | U251（胶质瘤细胞系） | U87（胶质瘤细胞系） |
| VT（人绒毛膜滋养层细胞） | 癌症干细胞 | 大鼠肝细胞 |
| 猴B细胞系 |
人外周血活化淋巴细胞 |
永生化人肌肉细胞 |
| 小鼠脾脏活化淋巴细胞 |
小鼠ES细胞系 |
人胃上皮细胞 |
| 大鼠神经祖细胞 | 大鼠脂肪细胞 | 狗肿瘤细胞 |
| 胶质瘤细胞系 | 牛脂肪细胞 | 牛子宫内膜上皮细胞 |
| 人扁桃体细胞 | 人肝细胞 | 人骨髓CD34+细胞 |
| 人巨噬细胞 | 人淋巴细胞 | 人输卵管上皮细胞 |
| 人胎儿卵巢成纤维细胞 | 人胎儿卵巢体细胞 | 人自然杀伤细胞 |
| 神经祖细胞 | 小鼠颅骨成骨细胞 | 心肌祖细胞 |
| 猪成纤维细胞 |
ES细胞（小鼠）使用实例
T.Hikichi,et al; Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer, Stem Cells, 2007, 25, 46-53
更多相关资料请点击文字：
◆产品列表
| 产品编号 | 产品名称 | 产品规格 |
| 302-14681 | BAMBANKER BAMBANKER冻存液 |
120 mL |
| 306-14684 | 20 mLx5 | |
| 306-95921 | BAMBANKER Direct BAMBANKER直接冻存液 |
20 mL |
※ 本页面产品仅供研究用。研究以外不可使用。
Bambanker® 与其他相关产品的比较
细胞冻存效果验证
细胞冻存液类型
１．Bambanker®
２．Medium with serum （含血清，A公司）
３．Serum-free Medium （无血清，A公司）
实验结果
*1：细胞-80℃的保存时间
◆Q＆A
| １. |
Q：为什么我使用了 BAMBANKER® 来保存细胞，但是存活率依然不高？ A：请冻存前确保细胞处于生长对数期，并且冻存时细胞数目控制在 5×105~1×107/mL 冻存液。 |
| 2. |
Q：我们实验室已经有固定的冻存程序了，换了你们的 BAMBANKER® 可以还继续用原来程序降温的方法冻存吗？ A：虽然本产品可以无需程序降温冻存细胞，但如果通过程序降温盒等适当控制了温度下降的速度，效果更佳。 |
| 3. |
Q：哪些细胞株（系）适合使用BAMBANKER® 来进行细胞冷冻保存？ A：几乎所有细胞株（系）都可以使用 BAMBANKER® 进行冻存。对于较为宝贵的 ES/iPS 细胞的保存尤其适用。官网上所列举的细胞系均已经过测试验证。 但也并不排除可能有某些细胞株是不适合使用 BAMBANER® 来进行冻存的，用户在没有确认是否可使用时，建议在进行正式细胞冻存之前先进行预实验。 |
| 4. |
Q：无血清冻存液相比传统含血清冻存液有什么优势？ A：无血清冻存液因不含有动物血清，质量更稳定，批间差小；同时未知生物成分或感染性物质污染细胞的几率也极低，尤其对于 ES/iPS 细胞等有可能用于再生医疗的细胞安全得以严格保障；可以直接冻存无血清培养的细胞，免去无血清再驯化的步骤；另外 BAMBANKER® 无血清细胞冻存液不需要像传统的血清冻存液需要程序降温，减少了用户的繁琐操作，节省了时间。 |
| 5. |
Q：BAMBANKER® 在冷冻保存细胞的过程中起什么作用？ A：BAMBANKER® 无血清细胞冻存液使用了 DMSO 等作为保护剂，在冻存细胞时能以１℃/min 左右的温度下降而逐渐冻结，在此过程中，细胞内的水分子被置换成冻结保护剂，抑制胞内和细胞周边的冰晶的形成，防止细胞膜和细胞器结构损伤，防止蛋白变质。 |
| 6. |
Q：未使用的BAMBANKER® 无血清细胞冻存液应该如何保存？ A：2-10℃ 避光保存。开封后尽快使用。请注意保质期为自生产日期起 24 个月。 |
| 7. |
Q：BAMBANKER® 能否使用于医疗领域? A：BAMBANKER® 仅供科研使用，不能使用于人体或医疗领域。 |
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- 作者
- 内容
- 询问日期
文献和实验
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