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文献和实验Preparation of denaturing 6% polyacrylamide gels for microsatellite analysis
between working with Repel-silane and Bind-silane a) Clean the plate with detergent (25% Lipsol) and warm water. (You may also need to use a razor blade to remove old bits of gels that have stuck). Rinse. b) Clean the upper surface
SSR GEL and Silver Staining Protocol
8 cm, and one lid Two rocking platforms Heat block for microtiter plates. A microplate vortexer is helpful. II. MOLD ASSEMBLY: Notes: Bind silane is toxic and should be used in a chemical fume hood. Wear gloves when handling this solution. Use
SSR GEL and Silver Staining Protocol
before working with bind silane and take care not to cross contaminate the plates with the two treatments. The acrylease treatment only needs to be repeated every four gels or so. 3. Prepare fresh 1 ml binding solution by making a solution 0.0005-0.001% bind
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