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APC anti-human CD271 (NGFR)参数介绍>>>>
| Clone | ME20.4 |
| Isotype | Mouse IgG1, κ |
| Reactivity | Human, Cross-Reactivity: Monkey, Raccoon, Sheep, Cat, Dog, Pig, Rabbit |
| Immunogen | WM245 melanoma cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody. |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change. " |
| Applications | FC - Quality tested |
| Application Notes | Additional reported applications for the relevant formats include: immunoprecipitation, Western blotting, immunohistochemical staining of frozen or paraffin embedded tissue sections, and blocking of NGF binding. |
| Application References | 1. Alonzo H, et al. 1984. P. Natl. Acad. Sci. USA 81:6681. |
| Description | CD271, also known as p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, and NGFR, is a type I transmembrane protein with a MW of 75 kD. It is expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells and melanocytes. The extracellular portion contains four TNFR-Cys repeats that form the binding domain for its ligands (NGF, BDNF, NTF3, and NTF4). The intracellular portion of CD271 contains a death domain, which interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9, to promote cell apoptosis, and to regulate cell differentiation and neurogenesis. |
| Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
品牌介绍：Biolegend公司自2002年成立以来，一直致力于为生物医药研究领域提供一流的，高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士，建立了一支雄厚技术背景的专业队伍，并通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。短短几年时间，Biolegend公司得到了飞速发展，已跻身全球最先进的抗体公司行列，并在2009年成为美国哈佛大家的独家抗体供应商！ Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂，用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中，Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比，为广大科研工作者所接受！同时，公司配备了一支经验丰富的技术服务队伍，为客户提供完美的配色方案及快速的售后处理服务！
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文献和实验Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody
Most T lymphocytes of the immune system differentiate within the thymus along the CD4/CD8 developmental pathway by a highly ordered process termed thymic selection ( 1 , 2 ). The maturation status of thymocytes is commonly assessed
Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody
Most T lymphocytes of the immune system differentiate within the thymus along the CD4/CD8 developmental pathway by a highly ordered process termed thymic selection ( 1 , 2 ). The maturation status of thymocytes is commonly
流式课堂 | 易被忽略的 CD4、CD8 共表达细胞——胸腺淋巴细胞
做过流式实验的同学，对 CD4、CD8 这两个指标都不会陌生。检测 CD4+ T 细胞和 CD8+ T 细胞分型，是流式中最常见的实验之一。 通常，我们会先检测 CD3，来圈总T淋巴细胞，再分别用 CD4 抗体和 CD8 抗体，来区分 CD4+ T 淋巴细胞（辅助或调节性T细胞）和 CD8+ T 淋巴细胞（细胞毒性 T 细胞）。 新生小鼠脾脏细胞 上图为新生小鼠脾脏细胞进行的 CD3-PE、CD4-FITC、CD8-APC 三色流式实验结果。从图中可以看出，在新生小鼠脾脏中，绝大
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