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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Fixation/Permeabilization Diluent
- 规格：
100ml
antibodies to transcription factors and nuclear proteins, such as
Foxp3 and Ki-67, as well as cytokines and chemokines. Also required
for proper intracellular staining with these antibodies is the
Fixation/Permeabilization Concentrate (cat. 00-5123) and the
Permeabilization Buffer (10X) (cat. 00-8333). Alternatively,
all buffers required for staining with these antibodies can be
purchased as a set in the Foxp3?transcription Factor Buffer Staining
Set (Fixation/Permeabilization and 10X Permeabilization) (cat. 00-5523).
Please see our Best Protocols section for more information.
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文献和实验Overview of Cell Fixation and Permeabilization
but also to provide information about newly characterized proteins and carbohydrates. This chapter will review some of the advantages and drawbacks of common chemical fixation and permeabilization methods used for immunolocalization at the level of light microscopy.
Fixation and permeabilization protocol 5/30/91 Mike Finney
Fixation and permeabilization protocol 5/30/91 Mike Finney This protocol works for all stages except dauers (which won't open) and hypoclorite-treated eggs (which disintegrate). Luckily, hypochlorite treatment and fixation
Bromodeoxyuridine Staining of Mammary Tissue
and incubate sections overnight at 4oC with anti-BUdR-POD antibody (Boehringer Mannheim Cat.# 1 585 860) diluted 1:15 in PGB diluent. 11. Rinse sections 3 x 5 min in PBS. 12. Incubate with unactivated DAB for 5 minutes followed by activated DAB for 10 minutes
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