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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
西宝生物
- 库存:
1 case
- 英文名:
OMNI-TRAY,PS,CELL CULT. TREATED W/LID
- 保存条件:
ROOM TEMPERATURE
- 规格:
LABWARE
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文献和实验Standardized Post‐Mortem Examination and Fixation Procedures for Mutant and Treated Mice
Mice (see Strategic Planning) 10% (v/v) neutral buffered formalin (equivalent to 4% [w/v] formaldehyde; see recipe
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
in each well. Using a multichannel pipette colonies are dispersed by pipetting up and down 40x in a circular motion being careful not to make bubbles as these cause cell death, check that colonies are dispersed. Cells are then transferred to a 96 well tray
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
(2 x 106 cells ) + 0.73 ml PBS To A add 15 ug linearised plasmid DNA, IMMEDIATELY prior to electroporation.. B no DNA 3. Using a Biorad Gene Pulser, (McMahon, A.P., and Bradley, A. (1990) Cell 62 1073-1085). "Zap" each curvette twice i.e
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