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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
QuantiSir Specific Gene Knockdown Quantification Kit For Epigenetic Regulators-HDAC7 (96 assays)
- 供应商:
上海西宝
- 规格:
96次分析
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文献和实验Real-Time qRT-PCR standard curves... efficiency is too h
Greetings, In my Real-Time qRT-PCR experiments, I employ the standard curve method for quantification of gene expression. However, standard curves seem to be a huge hit-or-miss procedure for me, even with genes that are well-established
INTRODUCTION The mechanism of RNA interference has emerged as a practical tool to study loss of function in many organisms. To make the method of gene knockdown suitable for mammalian cells, short
, et al. Dissecting comples epigenetic alterations in breast cancer using CpG island microarrays [J]. Cancer Res, 2001, 61(23): 8375-8380.[41]Huang T H, Perry M R, Laux D E. Methylation profiling of CpG islands in human breast cancer cells [J]. Hum Mol
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