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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
ROOM TEMPERATURE
- 英文名：
STD. GRAD BLUE TIP, 101-1000 µL BULK PACK, NON-STERILE
- 供应商：
西宝生物
- 库存：
10x100/box
- 规格：
LABWARE
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文献和实验samples from the thermal cycler and immediately place on ice, with an ice pack on top of the samples, for 1 min. Turn off the power supply. Adjust comb if it has been pushed out of the gel during pre-heating. Load 4 µl of each sample per well. TIP
Clonality - X Chromosome Inactivation Assay
of the resuspended DNA into a reaction tube. Add 1 µl REact buffer 2. Add 1 µl Hha I. Incubate overnight at 37°C. Incubate at 90°C for 10 min. Use 2 µl of this digested DNA for PCR (see below). Use 2 µl of the non-digested DNA from B18 above as a negative
of a 10% gel 4 ml H2O 3.3ml 30% acrylamide/bisacrylamide (29:1 mix) 2.5 ml1.5M Tris(PH 8.8) 10% SDS 100 µl 10% ammonium persulfate 100 µl TEMED 8 µl 2. Stacking gel: 4 ml 2.7ml water 1 ml 0.67ml 30% acrylamide/bisacrylamide (29:1 mix) 0.ml 1.0M
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