Permeabilization Buffer (10X)

eBioscience Permeabilization Buffer, in conjunction with eBioscience
Intracellular (IC) Fixation Buffer (Cat. No. 00-8222) or Foxp3
buffers (Cat. No. 00-5521) can be used to fix and permeabilize cells prior
to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear
antigens. These solutions have been specially formulated for reducing non-specific
staining of fluorochrome-labelled antibodies and increasing specific fluorescence
signal to noise ratios.
The Fixation Buffer is used first followed by Permeabilization Buffer. Subsequent
washing steps, antibody dilutions, and incubations after cell permeabilization
should be performed using the Permeabilization Buffer. The final washing step
and cell resuspension prior to running the sample is done using Flow Cytometry Staining Buffer.

The Permeabilization Buffer is supplied as a 10X concentrate. The 10X stock
should be diluted in distilled water to a 1X solution prior to use. The 1X
final working solution contains 0.1% saponin and 0.009% sodium azide.