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- Biolegend
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- 421002
- 2025年07月13日
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100 ml
Permeabilization Wash Buffer (10X)参数介绍>>>>
| Recommended Usage | For use in permeabilization, dilute Permeabilization Wash Buffer (10X) to 1X in DI water. Resuspend fixed cells in diluted Permeabilization Wash Buffer and centrifuge at 350 xg for 5-10 minutes, and repeat the same process for twice. It is recommended that the reagent be titrated for optimal performance for each application. Please see Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website. " |
| Applications | ICC, ICFC - Quality tested |
| Application Notes | The Permeabilization Wash Buffer (10X) may have crystallization or precipitation observed when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is a heavy precipitation observed after dilution to 1X working solution, it can be filtered to clarify the solution. |
| Application References | 1. Smeltz RB. 2007. J. Immunol. 178:4786. |
| Description | Permeabilization Wash Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Permeabilization Wash Buffer is used to permeabilize cells following fixation with Fixation Buffer (Cat. No. 420801). It is supplied as a 10X solution and should be diluted in deionized water prior to use. Permeabilization Wash Buffer has been formulated to have minimal effects on cells, reduce non-specific staining and enhance the signal to noise ratio. It can be used for antibody dilutions and cell washing during intracellular staining. |
| Storage | This permeabilization wash buffer solution should be stored at 4°C. Do not freeze. |
品牌介绍：Biolegend公司自2002年成立以来，一直致力于为生物医药研究领域提供一流的，高质量的流式抗体、试剂盒及其它相关产品。公司聚集了一批来自PharMingen的专业杰出人士，建立了一支雄厚技术背景的专业队伍，并通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以最合理的价格为广大客户提供卓越的免疫学和细胞生物学产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。短短几年时间，Biolegend公司得到了飞速发展，已跻身全球最先进的抗体公司行列，并在2009年成为美国哈佛大家的独家抗体供应商！ Biolegend公司的试剂主要包括细胞免疫、细胞因子、趋化因子、粘附分子等及与癌症研究、T 调节细胞研究、干细胞研究、先天免疫研究、细胞周期分析、凋亡研究及特定修饰类抗体等领域相关的试剂，用于流式、ELISA、免疫沉淀、WB、免疫荧光、免疫组化、及体内体外功能学试验。其中，Biolegend公司的流式抗体以其具有较宽范围的荧光选择及较高的性价比，为广大科研工作者所接受！同时，公司配备了一支经验丰富的技术服务队伍，为客户提供完美的配色方案及快速的售后处理服务！
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