20X SSPE BUFFER
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20X SSPE BUFFER
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3 Liters
525.9g NaCl sodium chloride
82.8g NaH2 PO4 sodium phosphate monobasic
28.2g EDTA powder FW=372
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bring up to 2400mls with H2 O
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add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)
- autoclave for 20 min
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2 Liters
350.6g NaCl sodium chloride
55.2g NaH2 PO4 sodium phosphate monobasic
18.8g EDTA powder FW=372
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bring up to 1600mls with H2 O
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add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)
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autoclave for 20 min
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1 Liters
175.3g NaCl sodium chloride
27.6g NaH2 PO4 sodium phosphate monobasic
9.4g EDTA powder FW=372
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bring up to 800mls with H2 O
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add NaOH to pH 7.4 (~27mls/liter of 10N NaOH)
- autoclave for 20 min
上一篇：10X TBE BUFFER 下一篇：0.5M EDTA
![SSPE [20X] (Sodium Chloride-Sodium Phosphate-EDTA) (0.2M phosphate buffer, 2.98M NaCl, 20mM EDTA, pH7.4)](https://img1.dxycdn.com/p/s14/2025/0305/454/4475752944173517981.jpg!wh200)
