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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Fixation/Permeabilization Concentrate
- 规格：
30ml
antibodies to transcription factors and nuclear proteins, such as Foxp3
and Ki-67 as well as cytokines and chemokines. Also required for proper
intracellular staining with these antibodies is the Fixation/Permeabilization
Diluent (cat. 00-5223) and the Permeabilization Buffer (10X) (cat. 00-8333).
Alternatively, all buffers required for staining with these antibodies can
be purchased as a set in the Foxp3/Transcription Factor Staining
Set (Fixation/Permeabilization and 10x Permeabiliation Buffer) (cat. 00-5523).
Please see our Best Protocols section for more information.
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文献和实验Overview of Cell Fixation and Permeabilization
but also to provide information about newly characterized proteins and carbohydrates. This chapter will review some of the advantages and drawbacks of common chemical fixation and permeabilization methods used for immunolocalization at the level of light microscopy.
Fixation and permeabilization protocol 5/30/91 Mike Finney
Fixation and permeabilization protocol 5/30/91 Mike Finney This protocol works for all stages except dauers (which won't open) and hypoclorite-treated eggs (which disintegrate). Luckily, hypochlorite treatment and fixation
1mL 细胞染色 buffer，300g 离心 5min，弃上清。 加入100μL 细胞染色 buffer，重悬细胞。 每管加入1mL 的 1× Fixation Working Solution，轻柔混匀，4℃ 避光孵育 30min。 600g 离心 5min，弃上清。 每管加入2mL 的 1×Permeabilization Working Solution，轻柔混匀。 600g 离心 5min，弃上清。 重复（6）、（7）步一次。 七、胞内抗体孵育 加入100μl 的1×
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