Fixation/Permeabilization Conc

Overview of Cell Fixation and Permeabilization

but also to provide information about newly characterized proteins and carbohydrates. This chapter will review some of the advantages and drawbacks of common chemical fixation and permeabilization methods used for immunolocalization at the level of light microscopy.

Fixation and permeabilization protocol 5/30/91 Mike Finney

  Fixation and permeabilization protocol 5/30/91 Mike Finney This protocol works for all stages except dauers (which won't open) and hypoclorite-treated eggs (which disintegrate). Luckily, hypochlorite treatment and fixation

细胞内细胞因子染色步骤

1mL 细胞染色 buffer，300g 离心 5min，弃上清。 加入100μL 细胞染色 buffer，重悬细胞。 每管加入1mL 的 1× Fixation Working Solution，轻柔混匀，4℃ 避光孵育 30min。 600g 离心 5min，弃上清。 每管加入2mL 的 1×Permeabilization Working Solution，轻柔混匀。 600g 离心 5min，弃上清。 重复（6）、（7）步一次。 七、胞内抗体孵育 加入100μl 的1×