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- 文献和实验
- 技术资料
- 抗体英文名：
Accumax - Cell Aggregate Dissociation Medium
- 规格：
100ml
cell counting, viral transfection assays, cell sorting, and flow
cytometry as well as bioreactor scale-up. Accumax has been shown
effective in dissociating cell clumps in a variety of cell lines
including hybridomas, CHO, BHK, 293, COS and Sf9 cells.
Notes:
1. The use of AccumaxTM may effect the integrity of certain antigenic
epitopes resulting in diminished antibody staining. This should be
determined experimentally for individual antibody clones.
2. Thaw Accumax at room temperature or 4C.
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文献和实验Neuronal Cell Dissociation Protocol
medium into 1 ml tube. Pipette 50 µl of cell-mix into tube of culture medium , and mix well. This is a counting sample of the final cell suspension.
Dissociation of Cells from Primary Tissue
实验概要 Obtain single cell suspensions from primary tissue. 主要 试剂 0.25% trypsin soybean trypsin inhibitor 主要设备 stainless steel mesh [Details：100
Single-Cell Enzymatic Dissociation of hESC Lines OxF1OxF4 and Culture in Feeder-Free Conditions
, with the inclusion of ROCK inhibitor in the medium. The protocols here describe the enzymatic dissociation of hESCs into a single-cell suspension and the plating of these cells onto either feeder cells or a protein-coated surface.
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