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Zymo Research货号E2001甲基化热启动酶-非预

混13611631389上海睿安生物
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  • ￥1148.01
  • Zymo Research
  • made in USA
  • E2001
  • 2026年01月14日
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    上海睿安生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保质期：

      1年

    • 英文名：

      ZymoTaq DNA Polymerase

    • 库存：

      100⁺支

    • 供应商：

      上海睿安生物13611631389

    • 保存条件：

      -20°C

    • 规格：

      50次/盒

    Zymo Research货号E2001甲基化热启动酶-非预混13611631389上海睿安生物

    Highlights

    • Hot-start DNA polymerase for robust product formation
    • Reduces non-specific PCR product formation from difficult templates (e.g., bisulfite converted DNA)
    • Compatible with real-time, quantitative PCR, and suitable for TA-cloning

    3小时完成修饰；利用热变性使未甲基化CpG位点的C变成G并采用离心柱纯化；得到的DNA可以直接用于下游实验。
    Zymo research 生物公司主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒，其DNA甲基化，羟甲基化，核酸提取等产品已得到世界各顶_级实验室的认可，产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出，独占鳌头。
    订购信息

    货号产品名称规格目录价格
    D5005DNA甲基化修饰试剂盒50次1694
    D5006DNA甲基化修饰试剂盒200次5630
    E2001甲基化热启动酶-非预混50次726
    E2002甲基化热启动酶-非预混200次2288
    D4001胶回收试剂盒50次570
    D4008胶回收试剂盒200次2008
    D6001土壤DNA提取试剂盒50次1920
    R2050RNA小量提取试剂盒50次1800
    D2001酵母质粒提取试剂盒（异丙醇沉淀法）100次950
    D2002酵母基因组提取试剂盒40次1230
    D2004酵母质粒提取试剂盒（离心柱法）50次1230
    T2001冷冻酵母转化试剂盒120次1164

    Product Description

     

    ZymoTaq DNA Polymerase contains all the reagents needed to perform “hot-start” PCR. The inclusion of a heat-activated, thermostable DNA polymerase reduces primer dimer and nonspecific product formation that can occur during PCR. This unique product is specifically designed for the amplification of bisulfite-treated DNA for methylation detection, but is applicable for conventional PCR. The product generates specific amplicons with little or no by-product formation. Simple and easy to use: Heat at 95°C for 10 minutes to initiate polymerization. ZymoTaq DNA Polymerase is a heat-activated, “hot start” polymerase that has 3′-terminal transferase activity. The addition of “A” overhangs to amplified DNA makes it ideal for use in TA-cloning.

     

    Technical Specifications

    Heat Activation95°C for 10 minutes
    Storage-20°C
    TemplatesDNA, bisulfite-converted DNA, CpG rich DNA

    产品细节图片1产品细节图片2

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    图标文献和实验
    该产品被引用文献

    Zymo Research货号E2001甲基化热启动酶-非预混13611631389上海睿安生物

    Highlights

    • Hot-start DNA polymerase for robust product formation
    • Reduces non-specific PCR product formation from difficult templates (e.g., bisulfite converted DNA)
    • Compatible with real-time, quantitative PCR, and suitable for TA-cloning

    3小时完成修饰；利用热变性使未甲基化CpG位点的C变成G并采用离心柱纯化；得到的DNA可以直接用于下游实验。
    Zymo research 生物公司主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒，其DNA甲基化，羟甲基化，核酸提取等产品已得到世界各顶_级实验室的认可，产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出，独占鳌头。
    订购信息

    货号产品名称规格目录价格
    D5005DNA甲基化修饰试剂盒50次1694
    D5006DNA甲基化修饰试剂盒200次5630
    E2001甲基化热启动酶-非预混50次726
    E2002甲基化热启动酶-非预混200次2288
    D4001胶回收试剂盒50次570
    D4008胶回收试剂盒200次2008
    D6001土壤DNA提取试剂盒50次1920
    R2050RNA小量提取试剂盒50次1800
    D2001酵母质粒提取试剂盒（异丙醇沉淀法）100次950
    D2002酵母基因组提取试剂盒40次1230
    D2004酵母质粒提取试剂盒（离心柱法）50次1230
    T2001冷冻酵母转化试剂盒120次1164

    Product Description

     

    ZymoTaq DNA Polymerase contains all the reagents needed to perform “hot-start” PCR. The inclusion of a heat-activated, thermostable DNA polymerase reduces primer dimer and nonspecific product formation that can occur during PCR. This unique product is specifically designed for the amplification of bisulfite-treated DNA for methylation detection, but is applicable for conventional PCR. The product generates specific amplicons with little or no by-product formation. Simple and easy to use: Heat at 95°C for 10 minutes to initiate polymerization. ZymoTaq DNA Polymerase is a heat-activated, “hot start” polymerase that has 3′-terminal transferase activity. The addition of “A” overhangs to amplified DNA makes it ideal for use in TA-cloning.

     

    Technical Specifications

    Heat Activation95°C for 10 minutes
    Storage-20°C
    TemplatesDNA, bisulfite-converted DNA, CpG rich DNA

    引用文献图片1引用文献图片2

    相关实验

    • Single tube confirmation PCR protocol

      results were obtained when 1 µl, 5 µl or 10 µl of the Zymo treated cells were used as template. 3. PCR reactions - The lyophilized confirmation primers (~5-10 nmoles of each oligonuleotide) should be resuspended in 750 µl of TE (final

    • Single tube confirmation PCR protocol

      as template for the colony PCR. However, fresher is probably better. *The amount of cells that gets picked into the Zymolyase does not seem to be too critical - similar PCR results were obtained when 1 µl, 5 µl or 10 µl of the Zymo treated cells were used

    • Single tube confirmation PCR protoco

      when 1 µl, 5 µl or 10 µl of the Zymo treated cells were used as template. 3. PCR reactions - The lyophilized confirmation primers (~5-10 nmoles of each oligonuleotide) should be resuspended in 750 µl of TE (final concentration of ~10 µM

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    Zymo Research货号E2001甲基化热启动酶-非预混13611631389上海睿安生物
    ￥1148.01