- ￥1372.01
- Zymo Research
- E1004
- Made in USA
- 2025年12月15日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
100⁺支
- 英文名：
Zymolyase
- 保质期：
1年
- 供应商：
上海睿安生物13611631389
- 保存条件：
-20°C
- 规格：
1000U/支
Zymo Research货号E1004[酵母裂解酶(冻干粉)]Zymolyase
上海睿安生物13611631389
HIGHLIGHTS
- 100 T Equivalent:Prepared from Arthrobacter lutes.Essential enzyme activities are β-1,3-glucanase and β-1,3-glucan laminaripentao-hydrolase.
- Convenient:Provided lyophilized along with a storage buffer for reconstitution.
- Efficient Cell Wall Digestion:Supplied storage buffer has been optimized to confer maximum levels of enzymatic activity.
DESCRIPTION
Digestion of yeast and fungal cell walls is necessary for many experimental procedures including spheroplasting, immunofluorescence, transformation, protein purification, and others. The use of lytic enzymes like Zymolyase is routinely used for digestion. The Zymolyase from Zymo Research is prepared from Arthrobacter luteus, lyophilized, and packaged with a resuspension buffer. The buffer has been optimized to confer maximal levels of enzymatic activity. The main activities of the enzyme are β-1,3 glucanase and β-1,3-glucan laminaripentao-hydrolase, which hydrolyze glucose polymers at the β-1,3-glucan linkages releasing laminaripentaose as the principal product. Optimal Zymolyase activity is at 30°-37°C; lytic activity ceases at higher temperatures.R-Zymolyase includes 0.5U/µl RNase A when reconstituted. Susceptible fungal genera:Asbya,Candida,Debaryomyces, Eremothecium, Endomyces,Hansenula, Hanseniaspora, Kloekera, Kluyveromyces,Lipomyces,Metschikowia,Pichia, Pullularia,Saccharomyces,Saccharomyces,Saccharomycopsis,Schizosaccahromyces,and Torulopsis.
13611631389上海雅裕生物&上海睿安生物
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文献和实验Zymo Research货号E1004[酵母裂解酶(冻干粉)]Zymolyase
上海睿安生物13611631389
HIGHLIGHTS
- 100 T Equivalent:Prepared from Arthrobacter lutes.Essential enzyme activities are β-1,3-glucanase and β-1,3-glucan laminaripentao-hydrolase.
- Convenient:Provided lyophilized along with a storage buffer for reconstitution.
- Efficient Cell Wall Digestion:Supplied storage buffer has been optimized to confer maximum levels of enzymatic activity.
DESCRIPTION
Digestion of yeast and fungal cell walls is necessary for many experimental procedures including spheroplasting, immunofluorescence, transformation, protein purification, and others. The use of lytic enzymes like Zymolyase is routinely used for digestion. The Zymolyase from Zymo Research is prepared from Arthrobacter luteus, lyophilized, and packaged with a resuspension buffer. The buffer has been optimized to confer maximal levels of enzymatic activity. The main activities of the enzyme are β-1,3 glucanase and β-1,3-glucan laminaripentao-hydrolase, which hydrolyze glucose polymers at the β-1,3-glucan linkages releasing laminaripentaose as the principal product. Optimal Zymolyase activity is at 30°-37°C; lytic activity ceases at higher temperatures.R-Zymolyase includes 0.5U/µl RNase A when reconstituted. Susceptible fungal genera:Asbya,Candida,Debaryomyces, Eremothecium, Endomyces,Hansenula, Hanseniaspora, Kloekera, Kluyveromyces,Lipomyces,Metschikowia,Pichia, Pullularia,Saccharomyces,Saccharomyces,Saccharomycopsis,Schizosaccahromyces,and Torulopsis.
13611631389上海雅裕生物&上海睿安生物
Single tube confirmation PCR protocol
(200mg/l). - Incubate at 30°C for two to three days. *This step reduces background by eliminating aborted transformants. 2. Zymolyase treatment - Transfer a small portion of a well-isolated colony (~1 mm) into 50 µl solution
Single tube confirmation PCR protocol
as template for the colony PCR. However, fresher is probably better. *The amount of cells that gets picked into the Zymolyase does not seem to be too critical - similar PCR results were obtained when 1 µl, 5 µl or 10 µl of the Zymo treated cells were used
Single tube confirmation PCR protoco
- Incubate at 30°C for two to three days. *This step reduces background by eliminating aborted transformants. 2. Zymolyase treatment - Transfer a small portion of a well-isolated colony (~1 mm) into 50 µl solution containing 60 U/ml
