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文献和实验SYBR Green Quantitative PCR Protocol
the relative values for your gene of interest’s change in expression. SYBR qPCR Quick Protocol StandardsØ Dilute the cDNA ~4-fold (e.g. 21μl sample + 59μl H2 O = 80μl)Ø Pool an appropriate amount from each sample to create standard 1 (i.e. 30μl x
Raft culture media (aka Green’s media)
Raft culture media (aka Green’s media) 3 parts DMEM (including glutamine or glutamax) 1 part Ham’s F12 5% FCS various supplements (not everybody uses the same supplements, some don’t use
Isolate DNA from NRBC Blood from Buccal Swab or dried blood spots collected with filter paper
mg/ml) into the sample. 2. Add 25ul OB Protease and 500ul Buffer BL into sample. Mix by vortexing at maxi speed for 30s. 3. Incubate at 65°C for 10 minutes. Collect any liquid drop from lid by brief centrifugation. 4. Centrifuge at 14
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