Orange Filter Cube (580/30)

Isolate DNA from NRBC Blood from Buccal Swab or dried blood spots collected with filter paper

mg/ml) into the sample. 2. Add 25ul OB Protease and 500ul Buffer BL into sample. Mix by vortexing at maxi speed for 30s. 3. Incubate at 65°C for 10 minutes. Collect any liquid drop from lid by brief centrifugation. 4. Centrifuge at 14

Hybridization of High Density Arrayed BAC Nylon Filter Blots

to keep wet. Place filter blots down on film (Kodak BioMax MS, 8326886) into X-Omatic Regular (Kodak) cassette with regular screens. Note: you may add special MS screens for increased sensitivity. Incubate -80°C, overnight. Leave cassettes

丁香园论坛关于显微镜问题汇总（一）

光显微镜，只有一个压制滤板（在目镜的下面，应该是压制滤板吧？），型号为0530。我要观察EGFP的荧光，其激发波为488nm，发射波为508nm，不知道能否观察？chujun_hust：压制滤板？应该是装filter cube的转盘，上面可以安装几个filter cube，每一种filter cube对应一种荧光燃料，这样可以同时观察几种荧光染料的荧光，不知道你实验室的是什么样的filter cube（excitation filter是什么类型的，DM是什么类型的，emission filter是什么类型