- ￥100 - 9999
- Bechman COULTER
- 356987
- 2025年07月12日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
).remove and save polylysine.wash plates 2x with 10ml sterile water.Seed cells: 1/2 X split from confluent plate (approximately 2-5 x 106 cells per 100mm plate) in PC12 culture media.Incubate overnight in 10% CO2 incubator.Cells should be 50-70% confluent
streptomycin, 37o C. 3. ECL matrix (E-C-L cell attachment matrix, Upstate Biotech, 08-110). Stored as 250 ul aliquots in 15 ml conical tubes at -80o C. Keep it cold all the time otherwise it will form a gel. 4. F12K medium (no additives), for diluting
of a 10x master solution: Grab a 500 ml conical flask Figure out how much of your dropout powder you''re going to need and weigh it out For example, the CSM - TRP mix we have is 0.74g/L, so for a 200ml of a 10x solution
技术资料暂无技术资料 索取技术资料
