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文献和实验Preparation of total RNA from whole blood collected in PAXgene tubes
Short PAXgene Protocol:spin in step 6 increased to 20 min; no Dnase treatment; optional wash after step 12 REQUIRED1.Centrifuge the PAXgene Blood RNA Tube for 10 min at 30005000 x g using a swing-out rotor.Note: Ensure that the blood sample
RNA was purified using the AllPrep DNA/RNA 96 Kit and analyzed on the Agilent 2100 bioanalyzer. Intact RNA is indicated by a RIN value of close to 10. RNA with no genomic DNA contamination. Total RNA was purified from rat kidney
temperature. Discard the flow-through. 10) Repeat Steps 8–9 once. 11) Centrifuge the spin cartridge at 12,000 × g for 1–2 minutes to dry the membrane with bound RNA. Discard the collection tube and insert the spin cartridge into a recovery
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