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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Human IL-10 ELISpot PLUS kit (HRP)
- 库存：
大量
- 供应商：
欣博盛
- 保质期：
见说明书
- 规格：
4 plates
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文献和实验Human ELISPOT Protocol Human ELISPOT Protocol（TNFa,IL13, IL12,IL10, IL6,IL5,IL4,IL2,IL1b,IFNg ,FasL） the protocol is in the file below:
, cryopreserving, and thawing human PBMCs; and finally, detecting vaccinia-specific IL-10 and IFNγ secreting lymphocytes, as well as CD8+ IFNγ T cells following in vitro stimulation of PBMCs with vaccinia virus. The methods presented below, although optimized
in the same volume of Buffer 1 as the initial volume transferred from the vial in step 2. 2. Sample Preparation Preparation of MNC from Buffy Coat to Obtain Low Platelet Numbers 1) Dilute 10 – 18 ml buffy coat with Buffer 2 to a total
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