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文献和实验RNA was purified using the AllPrep DNA/RNA 96 Kit and analyzed on the Agilent 2100 bioanalyzer. Intact RNA is indicated by a RIN value of close to 10. RNA with no genomic DNA contamination. Total RNA was purified from rat kidney
Preparation of total RNA from whole blood collected in PAXgene tubes
Short PAXgene Protocol:spin in step 6 increased to 20 min; no Dnase treatment; optional wash after step 12 REQUIRED1.Centrifuge the PAXgene Blood RNA Tube for 10 min at 30005000 x g using a swing-out rotor.Note: Ensure that the blood sample
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
on a clean adsorbent paper towel and air dry the pellet for 10-15 minutes. 14. Add 0.1 volume of Buffer EB and vortex for 1 minute to mix. 15. Incubate sample at 65°C for 1 hour to rehydrate DNA. Gently shake tube several times during incubation
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