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西宝生物
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文献和实验Preparation of total RNA from whole blood collected in PAXgene tubes
has been incubated in the PAXgene Blood RNA Tube for a minimum of 2 h at room temperature, in order to achieve complete lysis.Preheat a water bath or heat block (for microcentrifuge tubes) to 55℃, during first spin.We spin at 3750 rpm in a standard table
E.Z.N.A. Mag-Bind Blood DNA Protocol (1-100 ul Blood)
tubes or microplates 实验步骤 1. Add 1-100 ul blood sample to a nuclease-free microcentrifuge tube. Bring the volume up to 100 ul with Elution Buffer provided with this kit. 2. Add 100 ul Buffer MSL and 10 ul Proteinase K
RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysis
-wide transcriptome analysis. Cells, and thereby also RNA is immediately stabilized after the blood draw by a commercially available device (PAXgene). Total RNA is then extracted from PAXgene-stabilized blood and subjected to microarray analysis. In our hands
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