细胞周期甲基化芯片Cell Cycle DNA Methyl

Human Cell Cycle DNA Methylation PCR Array, Signature Panel: EAHS-201Z
The Human Cell Cycle EpiTect Methyl II Signature PCR Array profiles the promoter methylation status of a panel of 22 genes key to cell cycle regulation. Profiling cellular or fresh tissue genomic DNA samples with these arrays may help correlate CpG island methylation status with biological phenotypes. Inappropriate increases or decreases in the expression of cell cycle regulators by epigenetic mechanisms contribute to oncogenesis and senescence. Epigenetic regulation of cell cycle gene expression also occurs during development and the differentiation of a variety of cells from multi-potent progenitor cells to terminally differentiated non-dividing cells. This array contains genes that positively and negatively regulate the cell cycle, its phase transitions and checkpoints, DNA replication, and cell cycle arrest. The results may also help provide further insights into the molecular mechanisms and biological pathways behind cell cycle regulation. With a simple restriction enzyme digestion and real-time PCR, research studies can analyze the promoter methylation status of 22 different cell cycle regulators with this DNA methylation PCR array. The EpiTect Methyl II PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease. Both 96-well and 384-well ( 4 X 96 ) formats are available.

G1 Phase & G1/S Transition: CCND1, CCNE1, CDK4, CDKN1B (p27KIP1).
S Phase & DNA Replication: MCM2, MCM4.
G2 Phase & G2/M Transition: CCNB1, CDK5RAP1, CKS1B.
M Phase: CCNF, MRE11A, RAD51.
Cell Cycle Checkpoint & Cell Cycle Arrest: ATM, BRCA1, BRCA2, CDK2, CDKN1A (p21CIP1/WAF1), CDKN1B (p27KIP1), CHEK1, GADD45A, RAD9A, TP53.
Regulation of Cell Cycle: ATM, BRCA1, BRCA2, CCNB1, CCND1, CCNE1, CCNF, CDK2, CDK4, CDKN1A (p21CIP1/WAF1), CDKN1B (p27KIP1), CKS1B, GADD45A, RAD9A, RBL1, TP53