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- Liver miFinder 384HC
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- 2026年01月16日
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Liver miFinder 384HC
- 提供商：
SAB
Human Liver miFinder 384HC miRNA PCR Array: MIHS-3116Z
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The Human Liver miFinder 384HC miScript miRNA PCR Array profiles the expression of 372 miRNAs abundantly expressed or best characterized in liver tissue. As the number of annotated miRNAs continues to expand, it becomes increasingly important to focus only on those miRNAs expressed in a particular cell line or tissue of interest, as not all miRNAs are expressed in every tissue. We have experimentally analyzed the expression of miRNAs identified through miRBase V18 in a pool of human liver samples and correlated the results with scientific literature. Each of these miRNAs can regulate one or more messenger RNA transcripts, and conversely a given mRNA can be regulated by one or more miRNAs. Therefore, although they are well characterized, the complex role played by each miRNA is yet to be completely defined. This array maximizes the likelihood of discovering miRNAs whose expression patterns correlate with the biological phenotypes of liver tissue or hepatic cell lines under study. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green real-time PCR, the expression of a focused panel of miRNAs expressed in liver tissue can be easily and reliably analyzed with this miScript miRNA PCR Array. miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. Available in 384-well format. |
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文献和实验。一般是做6张可以了。但是还要有后继实验的啊，比如real time pcr，进行验证芯片结果。做几十例，然后做统计。 要是配对的样本做配对T检验，但先做F检验，看是正态还是偏态分布。然后选择合适的统计方法。 以上是个人意见。 lide658 你好：我也曾经做过PCR-array芯片，首先我觉得你实验组和对照组各做3张完全可以计算P值，其次一般认为有差异的倍数是3倍，若是条件放宽一点，2倍的也可以入选。对于结果分析方面，有些公司
Screening for miRNA Expression Changes Using Quantitative PCR (Q-PCR)
In this section, we describe the use of Applied Biosystems TaqMan Array microRNA Card Set 3.0 to identify miRNA expression in a given RNA sample. This array set includes an array “A” and an array “B” which each have 384 wells that contain
了。 因为有了扩增，TaqMan MicroRNA Assay就显得更加灵敏。研究人员只需要极少量的总RNA样品即可。DNA预扩增步骤的引进更显著降低了起始RNA样品量。即使1ng的总RNA，也能产生综合的表达图谱。 TaqMan MicroRNA Array则是将TaqMan MicroRNA Assay的所有优势集合并预装成便利的微流体芯片形式，特别适合人、大鼠和小鼠的图谱分析。它的原理其实很简单，就是384个定量PCR反应集中在一块微流量板上进行。每个阵列的内容与各自的Magaplex Primer
