泛素（泛素化）信号通路PCR芯片Ubiquitination

泛素（泛素化）信号通路PCR Array可以同时测定84个与泛素-蛋白酶体调控的蛋白降解相关的基因。干扰蛋白酶体的降解途径有可能引起多种疾病，例如癌症、糖尿病、心血管疾病和神经退行性疾病。芯片中含有泛素活化酶（E1）、泛素结合酶（E2）和泛素连接酶（E3）。这三种酶调控一些关键的细胞生物过程，如凋亡、细胞周期和转录。通过实时定量PCR的方法，研究者即能够利用该芯片简单可靠地同时检测泛素降解信号通路相关基因的表达。
Ubiquitin-Activating Enzymes (E1): ATG7, MOCS3, NAE1, NEDD8, SAE1, UBA1, UBA2, UBA3, UBA5, UBA6, UBE2Z. Ubiquitin-Conjugating Enzymes (E2): Regulators of Apoptosis: BARD1, BRCA1, TP53, UBE2Z. Regulators of Cell Cycle: ANAPC2, BARD1, BRCA1, CDC34, TP53, UBE2C, UBE2I. Regulators of Transcription: BRCA1, TP53, UBE2K, UBE2W. Other Ubiquitin-Conjugating Enzymes (E2): ARIH1, BTRC, NEDD8, PARK2, STUB1, TMEM189, UBA6, UBE2A, UBE2B, UBE2D1, UBE2D2, UBE2D3, UBE2E1, UBE2E2, UBE2E3, UBE2G1, UBE2G2, UBE2H, UBE2J1, UBE2J2, UBE2L3, UBE2M, UBE2N, UBE2Q1, UBE2R2, UBE2S, UBE2T, UBR2. Ubiquitin-Protein Ligases (E3): Regulators of Apoptosis: BARD1, BRCA1, CUL1, CUL2, CUL3, CUL4A, CUL5, TP53, UBE4B, VHL. Regulators of Cell Cycle: ANAPC11, ANAPC2, BARD1, BRCA1, CUL1, CUL2, CUL3, CUL4A, CUL4B, CUL5, CUL7, DDB1, MDM2, CUL9, SKP1, SKP2, TP53, VHL. Regulators of Transcription: BRCA1, CBL, MDM2, SMURF2, TP53, VHL, WWP1. Other Ubiquitin-Protein Ligases (E3): ARIH1, BRCC3, BTRC, DZIP3, FBXO3, FBXO31, FBXO4, FBXW10, FBXW9, HECW1, HECW2, HERC5, HUWE1, MARCH5, MIB1, MUL1, NEDD8, PARK2, RFWD2, RNF123, RNF148, SMURF1, STUB1, SYVN1, UBE2L3, UBE2T, UBR1, UBR2.